TABLE 4.

PCR amplification of ORFs from shared regions of divergence and adjacent flanking ORFs

RegionORFObservation of PCR productb
6006605860046010MAA10419698K10
aMAP0092+++++++++++++++++++++
MAP_RD1MAP0096c++++++++
MAP_RD1MAP0106c+++++++++++++++++++++
MAP0107+++++++++++++++++++++
MAP0845+++++++++++++++++++
MAP_RD2MAP0850c+++++++++++++++++
MAP_RD2MAP0866+++++++++++
MAP0867c+++++++++++++++++++++
MAP2146c+++++++
MAP_RD3MAP2158+++++++++++
MAP2164+++++++++++++++++++++
MAP_RD4MAP2189+++++++++++++
MAP2198+++++++++++++++++++++
MAP2749c+++++++++++++++++++++
MAP_RD5MAP2756c++++++++
MAP_RD5MAP2765c++++++++++
MAP2770+++++++++++++++++++++
MAP3715+++++++
MAP_RD6MAP3726++++++++
MAP_RD6MAP3751++++++++++++
MAP_RD6MAP3775c++++++++
MAP3783+++++++++++++++++++++
  • a —, ORF adjacent to divergent region and predicted to be present by microarray hybridization.

  • b +++, product observed; −, no product observed; +, weak product observed.