TABLE 2.

Proteins affected by YycF induction, as detected by proteomic analysis

Functional2 categoryProteinaSpot no.Ratio of inductionbTIGR4 no.Score (%)cCoverage (%)dpIMolecular mass (kDa)
5 min30 minLong-term
Fatty acid metabolismFabKe92.252.202.00Sp0419110465.1234.156
FabDe111.561.911.40Sp042095384.4533.184
FabGe152.392.571.84Sp0421207685.3425.739
FabFe61.491.741.75Sp042252135.4544.075
AccBe132.052.021.93Sp042353324.1717.023
AccCe41.341.441.57Sp0425123344.8549.793
AccAe162.742.511.80Sp042773186.3128.230
RegulationRReRR>16.20>39.22>33.08Sp1227171424.7824.265
RR*RR*>4.90>5.65>9.75Sp1227207484.7123.251
Purine and pyrimidine biosynthesisPurCe121.221.571.87Sp0044220664.6427.043
PurM71.321.151.24Sp004795274.4536.502
PurH21.391.211.62Sp0050162284.7556.316
PurB51.391.651.17Sp0056249545.0349.617
CarBe11.321.351.51Sp1275224274.52116.499
PyrR141.291.390.99Sp1278158565.1619.597
NrdF101.241.610.98Sp1180117374.1836.961
Central intermediary metabolismFTHFL31.421.811.65Sp1229134255.0959.806
Protein synthesisEF Tu81.981.861.30Sp1489197424.6043.943
StressGls24170.760.610.43Sp1804200694.3321.803
  • a AccA, acetyl-CoA carboxylase carboxyl transferase alpha subunit; AccB, acetyl-CoA carboxylase biotin carboxyl carrier protein; AccC, acetyl-CoA carboxylase biotin carboxylase; CarB, carbamoyl-phosphate synthase, large subunit; EF-Tu, translation elongation factor Tu; FabD, malonyl CoA-acyl carrier protein transacylase; FabF, oxoacyl-(acyl carrier protein) synthase II; FabG, 3-oxoacyl-(acyl carrier protein) reductase; FabK, enoyl-(acyl carrier protein) reductase; FTHFL, formate tetrahydrofolate ligase; Gls24, hypothetical general stress protein 24; NrdF, ribonucleoside diphosphate reductase 2, β subunit; PurB, adenylosuccinate lyase; PurC, phosphoribosyl-aminoimidazole succino-carboxamide synthase; PurH, phosphoriboxyl- aminoimidazole-carboxamide-formyltransferase/IMP cyclohydrolase; PurM, phosphoribosylformylglycinaamide cycloligase; and PyrR, pyrimidine operon regulatory protein.

  • b Ratios were calculated by dividing the mean values of the spot intensities (from three independent experiments) obtained for JNR7/87(pPL100) and JNR7/87 (pLS1RGFP). The mean values and standard deviations of the spot intensities are depicted in Table S2 in the supplemental material.

  • c The score is −10log (P), where P is the probability that the observed match is a random event.

  • d Coverage is the ratio of amino acids (no. identified in peptides/no. in theoretical peptides from sequence data) expressed as a percentage.

  • e Alterations of expression were also detected by microarray analysis.