TABLE 4.

Transfer frequencies of CTn341 mutations

StrainMutationModuleaTransfer frequencyb
With tetracyclineWithout tetracycline
IB399Wild type8.8 × 10−41.7 × 10−8
IB395Wild type9.1 × 10−5NDc
395-TAΔtraACon8.3 × 10−7ND
395-TDΔtraDCon<10−8ND
Jo37traGCon<10−8<10−8
Jo90traICon<10−8<10−8
399-TJΔtraJCon1.2 × 10−7ND
Jo22traKCon<10−8<10−8
Jo32traMCon<10−8<10−8
399-TOΔtraOCon2.9 × 10−4ND
Jo103traPCon1.0 × 10−59.7 × 10−8
399-TQΔtraQCon3.1 × 10−3ND
395-MAΔmobACon<10−8ND
Jo36mobBCon<10−8<10−8
Jo26mobCCon6.2 × 10−5<10−8
Jo68rteAReg<10−8<10−8
Jo69rteBReg<10−8<10−8
395R29ctn029Reg1.1 × 10−4ND
Jo97excDNA<10−8<10−8
Jo3bmhA1DNA8.7 × 10−51.0 × 10−8
Jo70bmhA2DNA6.6 × 10−7<10−8
Jo49iepDNA2.5 × 10−5<10−8
  • a The modules are the functional regions shown in Fig. 2 and 3. Con, conjugation; Reg, regulation and antibiotic resistance; DNA, DNA metabolism.

  • b Transfer frequencies were determined by determining the number of transconjugants per input donor cell and are means of three replicates. Cultures were either induced with tetracycline as indicated in text or not induced.

  • c ND, not determined.