TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    JS964MC1061 malP::lacIq Δmin::kan 28
    W3110PrototrophLab collection
    PS88W3110 Δmin::kanP1(JS964) × W3110, select for Kanr
Plasmids
    pJC106pBAD18::GFP (mut2) 10
    pAM239XmnI plus FnuD2 fragment of pUC19 containing the lac promoter in pGB2 8
    pJPB210 minCDE on pGB2 28
    pZH106PBAD::gfp-minD in pJC106 10
    pZH108Plac::gfp-minCDE 10
    pZH118PBAD::gfp-dicB on a pBAD18 derivativeZ. Hu and J. Lutkenhaus, unpublished data
    pZH119Plac::dicB in pAM239Z. Hu and J. Lutkenhaus, unpublished data
    pZH110 minC (pJPB210 ΔminDE) 10
    pJC22 minC in pGBT9 (BD-minC) 16
    pJC41 minD in pGAD424 (AD-minD) 16
    pJC51 dicB in pGAD424 (AD-dicB)Z. Hu and J. Lutkenhaus, unpublished data
    pZH112PBAD::malE-minC116-231 13
    pSEB12 minCDE in mini-F plasmid 28
    pHJZ109Plac::gfp-minC116-231minD 39
    pHJZ102PBAD::gfp-minC116-231This study
    pSEB104PBAD::ftsZ-gfp in a pGB2 derivative 29
    pCS104CDPBAD::minCD in a pGB2 derivative 41
    pJF118EHExpression vector with Ptac 11
    pHJZ117Ptac::minC in pJF118EHThis study