TABLE 2.

Features of CTn341 open reading frames

ORFDesignationCDSaProtein mol wtNo. of amino acidsbHomologc% Identity (no. of amino acids)dMotifePutative functionOrganismAccession no.f
ORF-1ctn0011056-1448f15,749131Hypothetical80 (71)B. thetaiotaomicronNP_811196
ORF-2ctn0021465-1890f16,386142
ORF-3ctn0031890-3170f49,482427Hypothetical20 (146)Enterococcus faecalisNP_817052
ORF-4ctn0043702-3947f9,44482
ORF-5yahA4004-4309f11,864102YahA35 (100)E. coliBAB12595
ORF-6ctn0064477-5109f24,422211
ORF-7lys5188-5712r20,287175Lys29 (153)COG3772 phage-related lysozyme (muraminidaseLysis proteinB. subtilis, phage B103NP_690649
ORF-8traQ5712-6215r19,064168TraQ86 (164)TransferB. thetaiotaomicronAAG17842
ORF-9traP6237-711232,943292TraP74 (254)pfam01807, CHC2 zinc fingerTransfer primaseB. thetaiotaomicronAAG17841
PrmN143 (288)COG0358 DnaG, DNA primaseBacteroides uniformisAF238307
ORF-10traO7124-7696r21,021191TraO94 (191)TransferB. thetaiotaomicronAAG17840
ORF-11traN7702-8685r37,114328TraN94 (329)TransferB. thetaiotaomicronAAG17839
ORF-12traM8757-10103r48,847449TraM85 (454)TransferB. thetaiotaomicronAAG17838
ORF-13traL10087-10392r11,764102TraL69 (102)TransferB. thetaiotaomicronAAG17837
ORF-14traK10401-1102124,028207TraK96 (207)TransferB. thetaiotaomicronAAG17836
ORF-15traJ11056-12057r37,164334TraJ90 (305)TransferB. thetaiotaomicronAAG17835
ORF-16traI12064-12690r24,168209TraI94 (209)TransferB. thetaiotaomicronAAG17834
ORF-17traH12720-13106r14,750129TraH85 (128)TransferB. thetaiotaomicronAAG17833
ORF-18traG13141-15822r103,339894TraG95 (834)COG3451, VirB4, type IV secretory pathwayTransferB. thetaiotaomicronAAG17832
ORF-19traF15642-15971r12,599110TraF96 (108)TransferB. thetaiotaomicronAAG17831
ORF-20traE15985-16293r10,654103TraE65 (105)TransferB. thetaiotaomicronAAG17830
ORF-21traD16497-17234r27,970246TraD72 (246)TransferB. thetaiotaomicronAAG17828
ORF-22traC17234-17593r13,942120TraC69 (120)TransferB. thetaiotaomicronAAG17828
TraB29 (65)TransferB. thetaiotaomicronAAG17827
ORF-23traB17620-18066r17,003149TraB79 (149)TransferB. thetaiotaomicronAAG17827
TraC30 (63)TransferB. thetaiotaomicronAAG17828
ORF-24traA18075-18827r28,277251traA94 (251)COG1192, Soj, ATPases, chromosome partitioningTransferB. thetaiotaomicronAAG17826
ORF-25mobA19509-19934f16,822142Hypothetical73 (138)B. thetaiotaomicronNP_812927
ORF-26mobB19916-21160f45,600415MocA-like93 (415)pfam03432, relaxaseTransfer and mobilizationB. thetaiotaomicronAAG17843
ORF-27mobC21194-23209f77,081672Putative Mob74 (667)pfam02534, TraG; COG3505, VirD4Transfer and mobilizationB. thetaiotaomicronAAG17844
ORF-28ctn02823347-26598r124,2191,084Hypothetical23 (278)Shewanella oneidensisNP_716343
ORF-29ctn02926618-27745r43,078376Hypothetical28 (376)COG1373, ATPase COG4565, CitB, response regulatorCoxiella burnetiiAAO91368
ORF-30rteC28050-28700r25,174217RteC96 (203)RegulationB. thetaiotaomicronAAA22922.1
ORF-31ctn03128894-29313r16,089140Hypothetical38 (55)SynechococcusAAN46185
ORF-32rteB29596-30915r76,229675RteB98 (641)COG2204, AtoC, response regulatorRegulationB. thetaiotaomicronAAA22921
ORF-33rteA30911-33226r87,098772RteA92 (771)COG0642, BaeS signal transduction histidine kinaseRegulationB. thetaiotaomicronAAA22920
ORF-34tetQ33229-35151r72,156641TetQ98 (641)COG0480, FusA, translation elongation factors (GTPases)Tetracycline resistanceB. thetaiotaomicronQ00937
ORF-35bmhA-2 (exon 2)35812-38574r99,481878BmhA65 (859)COG4646, DNA methylase; COG0553, HepA, DNA/RNA helicasesDNA methylaseB. thetaiotaomicronNP_811239
ORF-36matR (intron-encoded protein)38564-40360r70,285599MatR42 (599)COG3344, retron-type reverse transcriptase; pfam01348, type II, intron maturaseIntron splicingLactococcusQ57005
ORF-37bmhA-1 (exon1)40885-44073r120,3701,060BmhA45 (756)COG4646, DNA methylase; COG0286, HsdMDNA methylaseB. thetaiotaomicronNP_813660
ORF-38ctn03844066-44650r22,264195Hypothetical100 (195)B. thetaiotaomicronCAD24288
ORF-39exc44681-46765r77,600695Exc95 (695)COG0550, TopA, topoisomeraseExcisionB. thetaiotaomicronCAD24285
ORF-40ctn04046829-48397r59,798523Hypothetical92 (505)B. thetaiotaomicronCAD24284
ORF-41ctn04148421-48768r13,923116Hypothetical93 (116)B. theaiotaotmicronCAD24283
ORF-42ctn04248775-49134r14,057120Hypothetical53 (120)B. thetaiotaomicronCAD24282
ORF-43ctn04349336-49632f11,58999Hypothetical98 (99)B. thetaiotaomicronCAD24281
ORF-44ctn04449750-49998f8,43283Hypothetical88 (43)B. thetaiotaomicronCAC47936
ORF-45ctn04550053-50412r13,612120Hypothetical87 (120)HTH; COG3943, virulence proteinB. thetaiotaomicronCAC47922
RhuM28 (87)SalmonellaBAA89634
ORF-46int50427-51659r47,885411Int97 (411)COG4974, XerD, site-specific recombinaseIntegrationB. thetaiotaomicronCAC47921
  • a CDS is the coding region of the gene.

  • b Number of amino acids in the predicted protein.

  • c Most similar protein in the databases.

  • d Level of identity of the protein to the homolog for the number of amino acids indicated in parentheses.

  • e Motifs were obtained from the National Center for Biotechnology Information conserved domain database.

  • f Accession number of the most similar protein.