TABLE 2.

Effects of minC mutations on MinD and DicB interactions

MinC mutantPhenotypic assayaYeast two-hybrid assayb
MinD activation (pZH106/pZH110)DicB activation (pZH118/pZH110)MinC-MinDMinC-DicB
R133A+++− (0)+++ (49)
S134A+++++/−+++
D144A++++++++++++
E210A++++++++++++
E156A++ (1)− (0)
R170A++++++++ (69)+ (1)
R172A++++ (35)+ (6)
D180A
E193A++++++NDND
D205A++++++NDND
Wild type+++++++++ (63)+++ (48)
  • a +++, lethal; ++, severe filamentation; +, slight filamentation; −, no effect.

  • b +++, dark blue developed in 30 min; ++, dark blue developed in 1 to 2 h;+, blue developed in 3 to 4 h; +/−, pale blue developed overnight; −, no color change. The numbers in parentheses are Miller units determined from the liquid assay. ND, not determined.