TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant propertiesaReference or source
Strains
    B. thetaiotaomicron BT5482Rfr, no plasmids or known transmissible elements4
    B. thetaiotaomicron IB394Rfr Tpr, ΔthyA derivative of BT5482This study
    B. thetaiotaomicron BTΩpFD699Rfr Tcr Emr, independent transconjugant from EC100(pFD699) × BT5482 mating41
    B. thetaiotaomicron IB395Rfr Tcr ΔthyA, independent transconjugant from B. vulgatus CLA341 × IB394 matingThis study
    B. thetaiotaomicron IB399Rfr Tcr Emr ΔthyA, independent transconjugant from EC100(pFD699) × IB394 matingThis study
    B. fragilis 638RRfr, no plasmids or known transmissible elements31
    B. vulgatus CLA341Tcr Fxr, clinical strain containing CTn341 and Tn455529
    E. coli EC100F mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZ ΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 gaIU gaIK λ rpsLnupGEpicentre
    E. coli DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 gaIU gaIK λrpsL nupG tonAInvitrogen
Plasmids
    pFD699Emr Tcr (Apr), pFD670::CTn341 hybrid element that replicates as plasmid in E. coli and integrates as CTn in Bacteroides, 56 kb41
    pYT102Tcr (Cmr), suicide plasmid containing thyA, 8.2 kb2
    pYT102:FxFxr (Cmr), derivative of pYT102 in which tetQ was replaced by cfxA, 8 kbThis study
  • a The antibiotic resistance markers in parentheses Apr and Cmr, are expressed only in E. coli strains; Rfr, Tpr, Tcr, Emr, and Fxr determinants are expressed only in Bacteroides strains.