TABLE 1.

Strains and Plasmids

Strain or plasmidRelevant genotype or descriptionReference or source
Strains
    MC1000F araD139 Δ(ara-leu)7697 galU galK ΔlacX74 rpsL thi9
    MC1061F hsdR2 mcrA mcrB1 araD139 Δ(ara-leu)7696 ΔlacX74 galE15 galU galK16 rpsL thi6
    MC4100FaraD139 Δ(argF-lac)U169 prsL150 relA1 deoC1 rbsR fthD5301 fruA2542
    DHB3MC1000 ΔmalF3 Δ(phoA[PvuII/]) phoR16
    DHB4DHB3/F′ lac-pro laclq16
    MB68DHB4 ΔdsbALaboratory stock
    JAH143MC1061 ΔmalE444/F′ lacIq Kanr37
    DRH223JAH143 ΔtrxAThis study
    CFS456MC4100 Δara714 ΔtrxA37
    CFS459CFS456 ffh77This study
Plasmids
    pTRC99aIPTG inducible, high expressionPromega
    pDSW204pTRC99a, attenuated promoter43
    pDSW206pTRC99a, attenuated promoter43
    pCFS119pDSW204 + dsbAss37
    pCFS122pDSW204 + phoAss37
    pCFS123pCFS119 + trxA37
    pCFS126pCFS122 + trxA37
    pMO1pTRC99a + trxA with 5′ NheI siteThis study
    pMO2pDSW204 + trxA with 5′ NheI siteThis study
    pMO3pDSW206 + trxA with 5′ NheI siteThis study
    pMO15pMO2 + torTsThis study
    pMO16pMO2 + appAssThis study
    pDHSS0pMO2 + asmAssThis study
    pDHSS1pMO2 + sfmCssThis study
    pDHSS5pMO2 + fecBssThis study
    pDHSS9pMO2 + treAssThis study
    pDHSS11pMO2 + papJssThis study
    pDHSS13pMO2 + pcoEssThis study
    pDHSS14pMO2 + tolBssThis study
    pDHSS17pMO2 + flgAssThis study
    pDHSS18pMO2 + yraPssThis study
    pDHSS19pMO2 + artIssThis study
    pDHSS20pMO2 + yraIssThis study
    pDHSS21pMO2 + ccmHssThis study
    pDHSS22pMO2 + focCssThis study
    pDHSS24pMO2 + nikAssThis study
    pDHSS25pMO2 + ycfSssThis study
    pDHSS29pMO2 + livJssThis study
    pDHSS34pMO2 + agpssThis study
    pDHSS35pMO2 + traUssThis study
    pDHSS37pMO2 + ybcLssThis study
    pDHSS45pMO2 + livKssThis study
    pDHSS46pMO2 + artJssThis study
    pDHSS49pMO2 + btuFssThis study
    pDHSS60pMO2 + ecoTssThis study
    pDHSS63pMO2 + flgIssThis study
    pDHSS64pMO2 + fepBssThis study
    pDHSS66pMO2 + ansBssThis study
    pDHSS72pMO2 + mepAssThis study
    pDHSS76pMO2 + dsbCssThis study
    pDHSS80pMO2 + ivyssThis study
    pDH273pCFS119 + mature portion of dsbAThis study
    pDH275pCFS122 + mature portion of dsbAThis study
    pDH358pDSW204 + mature dsbA with 5′ NheI siteThis study
    pDH359pDH358 + sfmCssThis study
    pDH360pDH358 + treAssThis study
    pDH361pDH358 + pcoEssThis study
    pDH362pDH358 + tolBssThis study
    pDH378pDH358 + malEssThis study