TABLE 1.

Strains and plasmids

Strain or plasmidRelevant characteristicsSource or reference
Ralstonia eutropha
    H16SH+MBH+DSM 428, ATCC 17699
    HF160SHMBH+HoxI hoxF::Tn535, 47
    HF412SH+MBH+hoxIΔ67
HF424SHMBH hoxFUYHWΔ hoxGΔ41
Escherichia coli
    S17-1Tra+ recA pro thi hsdR chr::RP4-263
    JM109F′ traD36 lacIq, Δ(lacZ)M15 proA+B+/e14 (McrA) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17(rKmK+) relA1 supE44 recA182
    XL1-BluerecA thi hsdR1 supE44 relA1 lac(F′ proAB lacq lacZΔM15 Tn10[Tet])13
Plasmids
    pEDY309Tcr RK2 ori Mob+; promoterless lacZ gene31
    pVK101Kmr Tcr RP4ori37
    pCH2341.3-kb KpnI fragment of pGE15 in pTZ18R67
    pCH2912.9-kb HindIII-BamHI fragment of pGE15 in pTZ18RA. Tran-Betcke and B. Friedrich
    pCH45515-kb HindIII of pGE15 in pBlueKS+41
    pCH552hoxHYΔ in pCH45541
    pCH7810.9-kb XhoI fragment of pCH234This study
    pCH1084Derivative of pCH234 with a 937-bp SacII-EcoRI PCR product containing the Strep-tag II sequence at the 5′ end of hoxIThis study
    pCH1085Derivative of pCH291 with a 818-bp HindIII-NcoI PCR product containing the Strep-tag II sequence at the 5′ end of hoxFThis study
    pCH108611.8-kb BamHI fragment of pCH455 in pCH1085This study
    pCH108710.6-kb BamHI fragment of pCH552 in pCH1085This study
    pGE1514.6-kb HindIII fragment of pHG1 in pVK10169
    pGE151Derivative of pRK40434
    pGE346SHMBH+ hoxFUΔ41
    pGE371SHMBH+ hoxYHΔ41
    pGE4360.9-kb KpnI-HindIII fragment of pCH781 in pGE151This study
    pGE549Derivative of pGE15 containing the 1.4-kb KpnI fragment of pCH1084This study
    pGE550Derivative of pGE371 containing the 1.4-kb KpnI fragment of pCH1084This study
    pGE551Derivative of pGE346 containing the 1.4-kb KpnI fragment of pCH1084This study
    pGE55214.6-kb HindIII fragment of pCH1086 in pEDY309This study
    pGE55313.4-kb HindIII fragment of pCH1087 in pVK101This study