TABLE 2.

Oligonucleotides used in this study

OligonucleotideSequenceaApplication
pparB-egfp-fwCTTCTGGAGGGCGAGGACGAGGACGGGGACGCCGAGTCCCTGCCGGGCCCGGAGCTGCCGGGCCCGGAGGTGAGCAAGGGCGAGGAGCTGConstruction of egfp-aac3(IV)-oriT cassette
pegfp-apra-rvGAAGCAGCTCCAGCCTACATATGATCTAGAGTCGCGGCCG NdeIConstruction of cassette
pegfp-apra-fwCGGCCGCGACTCTAGATCATATGTAGGCTGGAGCTGCTTC NdeIConstruction of cassette
pparB-apra-rvAAAGCAAAGGGCGGACCGTGTTCCGGTATGCACCGGACATATGATTCCGGGGATCCGTCGACC NdeIConstruction of cassette
pHTH-apra-fwGAAGGACTTCAACTGCACGCATGACCAGCTGGCGGACCGTACGTAGGAACTTCATGAGCTCAGCC SnaBIIntroduction of mutation in parB HTH motif
pHTH-apra-rvCTTCAGCAGACGCAGGGTGTTGGACACCTGCGGGCGGGACTCGTACGTAAGCTCCATCAGCAAAAGGGG SnaBIIntroduction of mutation in parB HTH motif
  • a Boldface indicates introduced changes, and italics indicate the restriction site.