TABLE 1.

Strains and plasmids

Strain or plasmidDescriptionReference or source
E. coli strains
    CC118λpirCloning strain18
    DH5αCloning strain17
V. fischeri strains
    Wild-type ES114Isolate from an E. scolopes light organ3
    ainS mutant CL21ainS gene partially deleted and replaced by a chloramphenicol resistance (cat) marker22
    litR mutant PMF8litR gene inactivated by insertion of a kanamycin resistance marker (kanR)9
    luxI mutant VCW2G7luxI gene inactivated by a frameshift mutation22
    luxO mutant CL42luxO gene inactivated by insertion of a kanamycin resistance marker (kanR)22
    luxR mutant CL53luxR gene inactivated by insertion of a erythromycin resistance marker (ermR)21
    luxS mutant CL39luxS gene inactivated by insertion of a kanamycin resistance marker (kanR)21
    ainS luxI mutant CL24Double mutant carrying mutations as described above22
    ainS luxO mutant CL64Double mutant carrying mutations as described above22
    ainS luxS mutant CL41Double mutant carrying mutations as described above21
    LuxO(D47E) mutant CL59LuxO protein carrying a T-to-G point mutation resulting in an aspartate-to-glutamate substitution at position 47This study
Plasmids
    pCL145pEVS79 carrying a 1.8-kb V. fischeri ES114 DNA fragment with the luxO gene22
    pCL150pCL145 with LuxO mutated by an aspartate-to-glutamate substitution at position 47This study
    pDM104-6pVO8 carrying a flaA::lacZ transcriptional fusion48
    pEVS79Allelic exchange vector40
    pKV112pVO8 carrying the gfp gene under Plac controlK. Visick
    pKV31pVO8 carrying a 1-kb SalI-PvuII fragment with the intact luxR geneK. Visick
    pVO8V. fischeri cloning vector, camR and ermR43