TABLE 4.

Aerobic NO consumption activity of P. aeruginosa mutant strainsa

Strain (genotype)Plasmid (gene carried)NO consumption activity (nmol of NO/min/mg of protein)
PAO1 (wild type)59.5 ± 13.4
PDM2664 (fhp)1.5 ± 1.8
PFM4302 (fhpR)0.5 ± 0.3
RM495 (norCBD)52.5 ± 1.4
PDM2664 (fhp)pHA1512 (fhp+)122.8 ± 33.0
PFM4302 (fhpR)pHA1512 (fhp+)96.5 ± 22.5
  • a The activities were determined amperometrically. Strains were grown aerobically in LB medium supplemented with 3 mM SNP. IPTG (isopropyl-β-d-thiogalactopyranoside) was added at 1 mM for the cells carrying pHA1512. Values are means ± standard deviations from more than three independent cultures.