TABLE 3.

Effect of the fhpR and rpoN genes on transcription of the denitrification genesa

Strain (genotype)β-Galactosidase activity (Miller U)
pHA531 (nirS::lacZ)pHA533 (norC::lacZ)pHA1243 (nosR::lacZ)
NoneNitrite (5 mM)NoneNitrite (5 mM)NoneNitrite (5 mM)
PAO1 (wild type)7.7 ± 113,963 ± 44722 ± 2012,258 ± 1,26233 ± 231,422 ± 249
PFM4302 (fhpR)32 ± 213,316 ± 41334 ± 148,560 ± 2,80512 ± 131,300 ± 122
RN102 (rpoN)573 ± 308,632 ± 1,933362 ± 14414,374 ± 1,398168 ± 962,265 ± 269
  • a Strains were grown under oxygen-limiting conditions in synthetic medium supplemented (or not) with 5 mM sodium nitrite. Values are means ± standard deviations from three independent cultures.