TABLE 2.

Proteins subjected to stasis-induced carbonylation at the time when carbonyl content is maximal. Proteins in bold have been demonstrated to be substrates for DnaK (19).

Protein no.GeneSize (kDa)Protein name
1 rpoB 151 RNA polymerase β-subunita
2 purL 141 Phosphoribosyl formylglycinamide synthase
3 carA 119 Carbamoylphosphate synthase
4 aceE 100 E1 component of pyruvate dehydrogenase
5 metE 85 Tetrahydropteroyltriglutamate methyltransferase
6 fus 78 Protein chain elongation factor G
7 dnaK 69Molecular chaperone DnaK
8 aceF 66 E2 component of pyruvate dehydrogenase
9 ptsI 64Phosphoenolpyruvate-protein phosphotransferase
1061Polyamine-induced protein precursor
11 groEL 57Molecular chaperone GroEL
12 glnA 52 Glutamine synthetase
13 gltD 52Glutamate synthase
14 pyk 51Pyruvate kinase
15 gnd 49 6-Phosphogluconate dehydrogenase
16 icd 46Isocitrate dehydrogenase
17 glyA 46Serine hydroxymethyltransferase
18 serA 44 d-3-phosphoglycerate dehydrogenase
19 sucB 44Dihydrolipoamide succinyltransferase
20 fabB 43β-Keto-[acyl-carrier-protein] synthetase
21 tufA 42Protein chain elongation factor Tu
22 sucC 41Succinyl CoA ligase
23 znuA 34High-affinity zinc uptake system protein precursor
24 mdh 32Malate dehydrogenase
  • a Proteins that have been demonstrated to be substrates for DnaK (19) are shown in boldface characters.