TABLE 2.

Proteins subjected to stasis-induced carbonylation at the time when carbonyl content is maximal. Proteins in bold have been demonstrated to be substrates for DnaK (19).

Protein no.GeneSize (kDa)Protein name
1rpoB151RNA polymerase β-subunita
2purL141Phosphoribosyl formylglycinamide synthase
3carA119Carbamoylphosphate synthase
4aceE100E1 component of pyruvate dehydrogenase
5metE85Tetrahydropteroyltriglutamate methyltransferase
6fus78Protein chain elongation factor G
7dnaK69Molecular chaperone DnaK
8aceF66E2 component of pyruvate dehydrogenase
9ptsI64Phosphoenolpyruvate-protein phosphotransferase
1061Polyamine-induced protein precursor
11groEL57Molecular chaperone GroEL
12glnA52Glutamine synthetase
13gltD52Glutamate synthase
14pyk51Pyruvate kinase
15gnd496-Phosphogluconate dehydrogenase
16icd46Isocitrate dehydrogenase
17glyA46Serine hydroxymethyltransferase
18serA44d-3-phosphoglycerate dehydrogenase
19sucB44Dihydrolipoamide succinyltransferase
20fabB43β-Keto-[acyl-carrier-protein] synthetase
21tufA42Protein chain elongation factor Tu
22sucC41Succinyl CoA ligase
23znuA34High-affinity zinc uptake system protein precursor
24mdh32Malate dehydrogenase
  • a Proteins that have been demonstrated to be substrates for DnaK (19) are shown in boldface characters.