TABLE 1.

S. pneumoniae strains used in this study

StrainGenotype and constructionSource or reference
RxR36A derivativeRavin (29)
CP1200Rx but malM511 str-1Shoemaker and Guild (34)
CP1415CP1200 but comA negative by insertion of the ermAM cassette into the ClaI site of comAMorrison et al. (20)
CP1500hex nov-r1 bry-r str-1 ery-r1 ery-r2Cato and Guild (5)
EK100CP1415 but egb negative by transformation with genomic DNA from R262Steinmoen et al. (35)
EK4166EK100 but hirL::pEVP3 by transformation with plasmid DNASteinmoen et al. (35)
H1CP1415 but Novr by transformation with CP1500 DNASteinmoen et al. (35)
H2H1 but lytA::pEVP3 by transformation with plasmid DNASteinmoen et al. (35)
H3H1 but lytA::pEVP3 by transformation with plasmid DNASteinmoen et al. (35)
H5EK100 but lytC::pEVP3 by transformation with plasmid DNASteinmoen et al. (36)
EK4168EK4166 but ΔcbpD (Materials and Methods)This study
L1EK100 but cbpD::pEVP3 by transformation with plasmid DNAThis study
L3CP1415 but ΔcbpD (Materials and Methods)This study
L5L3 but qsrA::cbpD (see Materials and Methods for details)This study