TABLE 1.

Primers used for construction of mutants and lacZ fusion strains

PrimerSequence (5′-3′)Construct(s) for which used
comDE1TCTGGATCCGCAACCGACATCTC comDE mutants, comC mutants
comDE2GGACTCTAGACTCCAATCGCGGTATT comDE mutants, bsmA′-lacZ
comDE3GTCTTCCATGGTAGCTGCAATG comDE mutants
comDE4GATCCTGCAGTTACTGTCGTC comDE mutants
comC1TACCCTCGAGAATATCGGCCATC comC mutants
comC3CCGCCTGCAGTAGCCATATAACC comC mutants
comC5GTCCCATGGGTTTTTTTCATTTTATATCTCC comC mutants, bsmA′-lacZ
ciaRH1TGTAGGGATCCCCACTACAGATAC ciaRH mutants
ciaRH2GCTCAAGCTTTGCATGACATCAGC ciaRH mutants
ciaRH3GCTACCATGGAAGAGGTCAGAAAC ciaRH mutants
ciaRH4GAGCTGCAGACATTACTCCTTGTG ciaRH mutants
comX1GATCGGATCCTGATGTCGTAGGTTCG comX mutants
comX2GATCGAGCTCTTCACGAGTCCAC comX mutants
comX3CATGCCATGGGAGCGCTTGGTAGCA comX mutants
comX4GATCCTGCAGTGCAGCAGCATCAC comX mutants
comA1GATCGGATCCTCGTCATAGCCGTTA comA mutants
comA2GATCGAGCTCATGACCTGCTGGATA comA mutants
comA3GATCCCATGGTCTTGTCGAATCTGAA comA mutants
comA4GATCGAATTCCAGTACGCTGTGAA comA mutants
nlmAB1GATCGAATTCGATGTAATGGACAGCC nlmAB′-lacZ (OMZ1008 and derivatives)
nlmAB2AGTGTGGATCCACTACAGATCCAAC nlmAB′-lacZ (OMZ1008 and derivatives)
immA2AGAGGATCCTTGCGCAGCCAG immA′-lacZ
immA9GTAGGCATGCTGATTCTGCTGGCA immA′-lacZ
immB9GATCTCTAGATTGCACAAGCACGCTG immB′-lacZ
immB10GATCGCATGCAGCAGCTGAGAAG immB′-lacZ
bsmK1GATCGCATGCAACCTAGCCACTCTC bsmK′-lacZ
bsmK2GATCTCTAGATCCAGGGAGCGGAAA bsmK′-lacZ
bsmE1CTACTCTAGATATTGGCTGCTTTTACACT bsmE′-lacZ
bsmE2GATCGCATGCAACTTGCTGCACCTGA bsmE′-lacZ
bsmI1GATCTCTAGACGGCGAACTGCTGTA bsmHI′-lacZ
bsmI3GATCGCATGCTAACGTCTCTGTATCC bsmHI′-lacZ
nlmAB10CATGTCTAGAGGTGTAGAGGCTATTATGTAG nlmAB′-lacZ (OMZ1033)
nlmAB11GATCGCATGCTTCAAATGCCTGTGTA nlmAB′-lacZ (OMZ1033)
nlmAB-EcorV-fCATGGATATCTTAAGACAAAATAGCTACCA nlmAB′-lacZ with EcoRV site (OMZ1034)
nlmAB-EcorV-rCATGGATATCATTTTTGATGTTCTGAAACT nlmAB′-lacZ with EcoRV site (OMZ1034)
nlmAB-PstI-fATAGCTGCAGTTTAGGATATTTTGCTCTATTTTG nlmAB′-lacZ with PstI site (OMZ1035)
nlmAB-PstI-rGTACCTGCAGCTATTTTGTCTTAAACGGTC nlmAB′-lacZ with PstI site (OMZ1035)
nlmAB-ClaI-fCATGATCGATATTTTGCTCTATTTTGAAAA nlmAB′-lacZ with ClaI site (OMZ1036, OMZ1037)
nlmAB-ClaI-rAGCTATCGATTTTTGATGTTCTGAAAC nlmAB′-lacZ with deletion and ClaI site (OMZ1037)
nlmAB-ClaI-r2GATCATCGATAATGGTAGCTATTTTGTCT nlmAB′-lacZ with ClaI site (OMZ1036)