TABLE 2.

Plasmids

PlasmidInsert/constructionSource/reference
pTrc99AExpression vector, pBR322 ori, pTrc promoter, AprAmersham Pharmacia Biotech
pBA169pTrc99A ΔNcoI; eliminates vector ATG; AprAlba (unpublished)
pCO109yfgL in pBA169, AprThis study
pCO110yfiO in pBA169, AprThis study
pCO122nlpB in pBA169, AprThis study
pCO123yraP in pBA169, AprThis study
pCP20FLP+ λcI857+ λP Rep(Ts) Cmr Apr9
pKD3FRT-flanked Cmr gene from pSC140 cloned into pANTSγ, Cmr11
pKD4FRT-flanked Kmr gene from pCP15 cloned into pANTSγ, Kmr11
pKD46araC, ParaB—γ, β, and exo in pINT(Ts), Apr11