TABLE 1.

Bacterial strains and primers

Strain or primerGenotype, relevant characteristics, or nucleotide sequenceReference
Strains
    S. aureus
        DSM20231Wild-type standard laboratory strain (DSMZ) 41
        SH10008325-4 rsbU+ 21
        PBM001DSM20231 clpC::ermBThis study
        HCH001SH1000 clpC::ermBThis study
        MGM001PBM001(pCX19clpC)This study
        GP266RN4220 rsbU+sigB1(Am) Tcr 4
        MB290DSM20231 sigB1(Am) TcrThis study
        MB288PBM001 sigB1(Am) Ermr TcrThis study
        SA113Restriction-negative mutant of S. aureus 8325-4 23
    S. carnousus TM300Invasion-negative control strain 40
Primers
    Cloning
        MclpCForw5′-ATATGGATCCTGTAGAATGATAACTATGGAAGAGG-3′
        MclpC2Rev5′-ATATGGATCCCAAATTCACCACGATATTTAGTACC-3′
        clpC25′-ATATGGTACCTGGACTGTTTTATATTATGCTTGC-3′
        ermBFor5′-ATGAACAAAAATATAAAATATTCTCAAAAC-3′
        ermBRev5′-TTATTTCCTCCTCCCGTTAAATAATAGATAAC-3′
        KclpC1A5′-ATATGGATCCAGTAGGAGGTCATTATTTATGTTATT-3′
        KclpC2Sma5′-ATATCCCGGGTGGACTGTTTTATAATTATGCTTGC-3′
    Real-time RT-PCR
        acsA f15′-AAGATATGCAACGGTTATCTAATAAAGCA-3′
        acsA r15′-AGGTGTACGCGACATAAATATAAATACTCT-3′
        asp23 f15′-CAAGAACAAAATCAAGAGCCTCAAT-3′
        asp23 r15′-CTTCACGTGCAGCGATACCA-3′
        citB f15′-CATTACCACAAGGCGCAACA-3′
        citB r15′-GAACTCCACAAATTTACCAACAACA-3′
        clpB f15′-AGTAGCAGTTAGTGAGCCTGATG-3′
        clpB r15′-TCTATCTTGAATACGCACACCATG-3′
        clpC f15′-GAAGAAGCAATTCGTTTAAATCATTCA-3′
        clpC r15′-CTTTCTAATACTTTTGCAGCAATTCCTT-3′
        clpP f15′-TGACAACGTAGCAAATTCAATCGTAT-3′
        clpP r15′-CACTTCCACCTGGTGAATTAATGTAT-3′
        gyrB f15′-GACTGATGCCGATGTGGA-3′
        gyrB r15′-AACGGTGGCTGTGCAATA-3′