TABLE 1.

PCR primers and conditions for identification of potential virulence genes

PCR productOligonucleotide sequences (5′-3′)GenBank accession no.Position in genePositive controlPCR conditionsReference
fnbAFw, CAC AAC CAG CAA ATA TAGAJ629121424-4418325-41 min at 94°C, 1 min at31
Rv, CTG TGT GGT AAT CAA TGT C1785-1667    50°C, 2 min at 72°C
Fw, GGT AAT CAT TCA TTC GAG2355-23728325-441
Rv, TGG CAC ACT GTC GAA GTC2561-2544
clfAFw, GTA GGT ACG TTA ATC GGT TZ18852368-386Newman1 min at 94°C, 1 min at31
Rv, CTC ATC AGG TTG TTC AGG1951-1934    45°C, 2 min at 72°C
Fw, GAT TAA GCT TTA CGT TCA AC1805-1824Newman23
Rv, GAT TGG TAC CAT TTT TAG GTG2948-2928
clfBFw, TGC AAG ATC AAA CTG TTC CTAJ224764425-444Newman1 min at 94°C, 1 min at31
Rv, TCG GTC TGT AAA TAA AGG TA1020-1001    45°C, 2 min at 72°C
Fw, AGG ACA ATC GAA CGA TAC AAC G162-183Newman1 min at 94°C, 1 min at32
Rv, ACT ACG TAC AGC TCT CGT TCT AAC ACT618-592    52°C, 1 min at 72°C
cnaFw, AGT GGT TAC TAA TAC TGM817361719-1735Phillips1 min at 94°C, 1 min at31
Rv, CAG GAT AGA TTG GTT TA3457-3441    55°C, 2 min at 72°C
Fw, ATG GTA CCA AGA AGA TAC G688-705Philips30
Rv, TCT TGA TAC CAA GCT TGT G1052-1034
sdrEFw, CAG TAA ATG TGT CAA AAG AAJ005647650-668Isolate 4761 min at 94°C, 1 min at31
Rv, TTG ACT ACC AGC TAT ATC1416-1399    45°C, 1 min at 72°C
Fw, CTG AAA ACA CTA GTA CAG AAA ATG CA158-182Isolate 47616
Rv, GGT ACT GTT AAA CCT GAA GAA AAG1795-1818
ebpSFw, CAA TCG ATA GAC ACA AAT TCU4882640-59Isolate 2521 min at 94°C, 1 min at31
Rv, CAG TTA CAT CAT CAT GTT TA565-546    50°C, 1 min at 72°C
Fw, CGT CAA TCG ATA GAC ACA AAT37-57Isolate 25229
Rv, CTG TAC CAG CAC CAA TT638-621
tstFw, AAG CCC TTT GTT GCT TGC GAY07488136-54Mu 501 min at 94°C, 1 min at3
Rv, ATC GAA CTT TGG CCC ATA CTT T480-459    55°C, 2 min at 72°C
Fw, ACC CCT GTT CCC TTA TCA TC88-107Mu 5024
Rv, TTT TCA GTA TTT GTA ACG CC394-375
etaFw, GCA GGT GTT GAT TTA GCA TTM17357719-738Isolate D722 min at 94°C, 2 min at24
Rv, AGA TGT CCC TAT TTT TGC TG811-792    57°C, 2 min at 72°C
Fw, ACT GTA GGA GCT AGT GCA TTT GT308-330Isolate D7215
Rv, TGG ATA CTT TTG TCT ATC TTT TTC ATC AAC496-467
etbFw, ACA AGC AAA AGA ATA CAG CGM17348509-528Isolate I 1282 min at 94°C, 2 min at24
Rv, GTT TTT GGC TGC TTC TCT TG734-719    57°C, 2 min at 72°C
Fw, CAG ATA AAG AGC TTT ATA CAC ACA TTA C574-661Isolate I 12815
Rv, AGT GAA CTT ATC TTT CTA TTG AAA AAC ACT C1183-1153
pvl (lukF)Fw, ATC ATT AGG TAA AAT GTC TGG ACA TGA TCC AAB186917579-609Isolate D481 min at 94°C, 1 min at33
Rv, GCA TCA AST GTA TTG GAT AGC AAA AGC2072-2046    55°C, 2 min at 72°C
pvl (lukS)Fw, GCA AGG TTT TAT CAA TTC AAA GAC TAC TT234-262Isolate D4827
Rv, GGG TCA TTT GTT TTG AGA CCA ATA T344-320