TABLE 5.

EOP of various deletion mutant strains ± complementing plasmids on selected agents

StrainEOPa
3.5% SDS5 μg/ml rifampicin150 μg/ml novobiocin
WT0.90.10.7
ΔyfgL0.4*6 × 10−90.3*
ΔyfgL + pyfgLbNT1NT
ΔnlpB0.84 × 10−50.7
ΔnlpB + pnlpBbNT0.4NT
ΔyraP4 × 10−70.60.8
ΔyraP + pyraPb1NTNT
surA::Cm2 × 10−61 × 10−86 × 10−5
  • a Values for EOP are the averages of three determinations; almost all values differed from the mean by less than 50%. An asterisk indicates that colonies were not apparent until 48 hr at 30°C. For comparison, all other strains formed colonies after 18 hr at 30°C. NT, not tested.

  • b All complementation experiments were performed in the presence of IPTG to induce expression.