TABLE 4.

P1 transduction experiments demonstrate that providing YfiO is not sufficient to relieve σE essentiality

RecipientDonor P1(rpoE::Cm; nadB::Tn10)Donor P1(nadB::Tn10)
Number of Tetr transductantsNumber of Tetr Cmr cotransductantsLinkage (%)Number of Tetr transductants
MG1655 + pyfiO300>100
MG1655500>100