TABLE 4.

Phosphate-buffered NYB largely prevents cell death of P. aeruginosa strains growing individually

Strain and genotypeGrowth mediumaIncubation time (h)Cell density (OD600)Lysis (%)Viable countb (107 CFU/ml)Death (%)
PAO1 wild typeNYB245.38 ± 0.29(0)c970 ± 156(0)
481.35 ± 0.1175160 ± 2184
PAO6395 ΔlasRNYB243.93 ± 0.15(0)753 ± 65(0)
482.90 ± 0.1526288 ± 3062
PAO1 wild typeNYB-Pi244.78 ± 0.21(0)987 ± 123(0)
483.29 ± 0.2931737 ± 7125
PAO6395 ΔlasRNYB-Pi245.10 ± 0.22(0)1,443 ± 132(0)
483.49 ± 0.13261,103 ± 5924
  • a NYB-Pi is NYB buffered with 100 mM K phosphate, pH 7.0.

  • b Viable counts were determined by plating on nutrient agar.

  • c (0), 1% lysis evident (baseline).