TABLE 2.

Strains and plasmids used

Strain or plasmidGenotype or descriptionSource or referencea
Strains
    WM54 E. coli K-12 ΔlacX74Bill Metcalf
    JLD271WM54 sdiA271::CamWanner mutagenesis, deleting nt 8137 to 8856 of accession no. AE000284 and replacing with Camr of pKD3 (4)
    PAO1Wild-type P. aeruginosa 9
    14028Wild-type S. enterica serovar TyphimuriumAmerican Type Culture Collection
    BA61214028 sdiA1::mTn3 2
Plasmids
    pSB377 luxCDABE transcriptional fusion vector, Ampr ColE1 origin 24
    pSB401 luxR + luxI::luxCDABE; Tetr p15A origin 23
    pSB1075 lasR + lasI::luxCDABE; Ampr ColE1 origin 23
    pSB536 ahyR + ahyI::luxCDABE; Ampr ColE1 origin 20
    pTIM244 ahyI::luxCDABE; Ampr ColE1 originPCR with pSB536 template and oligos BA440 and BA441; cloned into pSB377/EcoRI
    pAL101 rhlR + rhlI::luxCDABE; Tetr p15A originPCR with PA01 template and oligos BA983 and BA985; cloned into pSB401/EcoRI
    pAL102 rhlI::luxCDABE; Tetr p15A originPCR with PA01 template and oligos BA984 and BA985; cloned into pSB401/EcoRI
    pAL103 luxR + luxI::luxCDABE; Tetr p15A originPCR with pSB401 template and oligos BA988 and BA962; cloned into pSB401/EcoRI
    pAL104 luxI::luxCDABE; Tetr p15A originPCR with pSB401 template and oligos BA961 and BA962; cloned into pSB401/EcoRI
    pAL105 lasR + lasI::luxCDABE; Tetr p15A originPCR with PA01 template and oligos BA980 and BA982; cloned into pSB401/EcoRI
    pAL106 lasI::luxCDABE; Tetr p15A originPCR with PA01 template and oligos BA981 and BA982; cloned into pSB401/EcoRI
  • a Abbreviations: nt, nucleotides; oligos, oligonucleotides.