TABLE 3.

Summary of quorum-sensing-related genes that were down regulated in (i) isolate LES400 compared to strain PAO1, (ii) isolate LES431 compared to strain PAO1, and (iii) isolate LES431 compared to isolate LES400

ORFaGene nameLB LES400 ↓ vs PAO1H2O2 LES400 ↓ vs PAO1LB LES431 ↓ vs PAO1H2O2 LES431 ↓ vs PAO1LB LES431 ↓ vs 400H2O2 LES431 ↓ vs 400Product description
QS activated
    PA00264.3Hypothetical protein
    PA0105coxB6.6Cytochrome c oxidase subunit II
    PA010714Conserved hypothetical protein
    PA1317cyoA13Cytochrome o ubiquinol oxidase subunit II
    PA1319cyoC4.56Cytochrome o ubiquinol oxidase subunit III
    PA14044.8Hypothetical protein
    PA1431rsaL35Regulatory protein RsaL
    PA1432lasI12Autoinducer synthesis protein LasI
    PA23653.2Conserved hypothetical protein
    PA2445gcvP23.82.9Glycine cleavage system protein P2
    PA2446gcvH23.92.2Glycine cleavage system protein H2
    PA31819.72-Keto-3-deoxy-6-phosphogluconate aldolase
    PA3182pgl2.66-Phosphogluconolactonase
    PA3183zwf5.92.1Glucose-6-phosphate 1-dehydrogenase
    PA31887426Probable permease of ABC sugar transporter
    PA3190469.63834Probable binding protein component of ABC sugar transporter
    PA3195gapA6.0Glyceraldehyde 3-phosphate dehydrogenase
    PA33694.0Hypothetical protein
    PA3418ldh6.4Leucine dehydrogenase
    PA36913.6Hypothetical protein
    PA36924.5Probable outer membrane protein precursor
    PA39235.9Hypothetical protein
    PA41317.2Probable iron-sulfur protein
    PA41334.1Cytochrome c oxidase subunit (cbb3 type)
    PA43115.7Conserved hypothetical protein
    PA44968.14.92.511Probable binding protein component of ABC transporter
    PA449814343.131Probable metallopeptidase
    PA4876osmE8.0Osmotically inducible lipoprotein OsmE
    PA48803.3Probable bacterioferritin
    PA491612Hypothetical protein
    PA49175.0Hypothetical protein
    PA50279.5Hypothetical protein
QS repressed
    PA0509nirN4.412Probable c-type cytochrome
    PA051040Probable uroporphyrin-III c-methyltransferase
    PA05128.41517Conserved hypothetical protein
    PA15593.9
    PA2007maiA18Maleylacetoacetate isomerase
    PA2008fahA19Fumarylacetoacetase
    PA2009hmgA7.8Homogentisate 1,2-dioxygenase
    PA2259ptxS2.85.1Transcriptional regulator PtxS
    PA22604.3Hypothetical protein
    PA226111Probable 2-ketogluconate kinase
    PA25404.8Conserved hypothetical protein
    PA31743.2Probable transcriptional regulator
    PA32052.6Hypothetical protein
    PA3364amiC244.612Aliphatic amidase expression-regulating protein
    PA33656.65.75.110Probable chaperone
    PA3391nosR1338Regulatory protein NosR
    PA3392nosZ62234256Nitrous oxide reductase precursor
    PA3393nosD5.94.67.3NosD protein
    PA3394nosF8.1NosF protein
    PA35752.7Hypothetical protein
    PA36624.27.71220Hypothetical protein
    PA3790oprC9.7377.4Putative copper transport outer membrane porin OprC precursor
    PA3872narI2.8Respiratory nitrate reductase gamma chain
    PA3877narK19.1Nitrite extrusion protein 1
    PA3913104.79.7Probable protease
    PA4442cysN2.3ATP sulfurylase GTP-binding subunit/APS kinase
    PA4587ccpR103.65.64412Cytochrome c551 peroxidase precursor
    PA4770lldP2.9l-lactate permease
    PA49183.24.535438.3Hypothetical protein
  • a These genes have been reported previously as being regulated by quorum sensing (either QS activated or QS repressed) (43, 51).