TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionaSource or reference
Strains
    E. coli JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZΔM15]Takara
    E. coli BLR(DE3)/pLysSFompT hsdSB(rB mB) gal dcm Δ(srl-recA)306::Tn10(Tcr) (DE3)/pLysS(Cmr) Novagen
    W. eutropha H16Wild typeATCC 17699
    W. eutropha TK0120ΔphaZc, derived from H16This study
    W. eutropha H16DZbc1ΔphaZb ΔphaZc, derived from TK0120This study
    W. eutropha OH1ΔphaZc, derived from H16 10
Plasmids
    pUC19Cloning vector; AmprTakara
    pET23bExpression vector; AmprNovagen
    pE3ReZcpET23b carrying amplified NdeI-XhoI fragment containing phaZcThis study
    pETOHpET23b carrying amplified NdeI-XhoI fragment containing phaZb 10
    pET171HpET23b carrying amplified NdeI-XhoI fragment containing phaZl 19
    pLO3Suicide vector; TcrsacB RP4 oriT ColiE1 ori 13
    pLO3dmZcpLO3 carrying N-terminal 295 bp and C-terminal 296 bp of phaZcThis study
    pJPPOHSuicide vector; carrying 306-bp fragment of phaZb; Kmr 10
  • a Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Tcr, tetracycline resistant.