TABLE 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidRelevant propertyaReference(s) or source
E. coli JM109Cloning host for pUC19ery and its derivativesPromega
L. plantarum WCFS1Wild type; single colony isolate from human saliva isolate NCIMB882617
L. plantarum WCFS1::pLAMA-K28L. plantarum WCFS1 with single chromosomal integration of pLAMA-K28This work
L. plantarum MSΔlamADCO mutant in L. plantarum WCFS1 with lamA replaced by lamA::tag gene replacement cassetteThis work
L. plantarum NCIMB8826 Int-1Emr, stable nisRK integrant in the tRNASer locus, after transformation with pMEC1041
L. plantarum MS18048Emr Cmr; L. plantarum NCIMB8826 Int-1 containing pNZ8048This work
L. plantarum MSI011Emr Cmr; L. plantarum NCIMB8826 Int-1 containing pNICE011This work
pUC19eryAmpr Emr; 3.8-kb derivative of pUC19 containing 1.1-kb HinPI fragment of pIL253 carrying the Eryr gene57
pLAMA-K28pUC19ery vector carrying lamA::tag gene replacement cassetteThis work
pNZ8048A derivative of the pNZ8030 series containing PnisA, a MCS, and TpcpN. NICE vector, Cmr9, 41
pNICE011lamBD translationally fused (NcoI) to pNZ8048, CmrThis work
pBACe3.6Bacterial artificial chromosome plasmid
  • a Emr, erythromycin resistant; Cmr, chloramphenicol resistant; Ampr, ampicillin resistant; DCO, double crossover; MCS, multiple cloning site.