TABLE 2.

Purification of PhaZc from E. coli harboring pE3ReZca

StepProtein (mg)Total activity (U)bSp act (U/mg)Yield (%)
Crude extract1702,50015100
Toyopearl ether-650M7.81,10014044
Toyopearl DEAE-650M2.990031036
  • a Enzyme was purified from 2 g (wet weight) of E. coli cells harboring pE3ReZc as described in Materials and Methods.

  • b 3HB pentamer was used as a substrate to measure the activity. One unit of enzyme activity catalyzed the formation of 1 μmol of 3HB per min.