TABLE 4.

Real-time RT-PCR analysis of expression of genes regulated by ArlR

N315 ORFN315 geneN315 descriptionChange in expression level (n-fold)a
RT-PCRMicroarray
SA0123Hypothetical protein42.822.1
SA0319Hypothetical protein5.14.7
SA1637lukDLeukotoxin LukD21.9
SA1882kdpDTwo-component sensor kinase3.12.9
SA1993lacFPTS system, lactose-specific IIA component176.5
SA1844agrATwo-component response regulator−5.7−2.8
SA0250lytSTwo-component sensor kinase−2.8−3.1
SA0252lrgAHolin-like protein LrgA−64.8−16.5
SA0270ssaASecretory antigen precursor SsaA−1.7−2
SA1583rotRepressor of toxin−4−1.9
  • a Normalized values in the arlR mutant over values in the wild-type strain. Negative numbers denote up-regulation in the wild-type strain.