TABLE 1.

Plasmids and strains used in this study

Plasmid or strainDescription or genotypeSource or reference
Plasmids
    pIC133mini-Tn10-OS delivery vector, Ermr, Spcr22
    pJM103Ampr, Cmr20
    pJM115amyE integration vector for lacZ fusions, Ampr Kanr2
    pJM117pMUTIN 4 derivative, Ampr CmrM. Perego, unpublished
    pJM134Spcr gene in pBluescript (Stratagene) Ampr SpcrM. Perego, unpublished
    pMA5B. subtilis/E.coli shuttle vector3
    pJV211pJM103-′phoA3
    pJV217pMA5-kapBC26P′-′phoA3
    pJC11pJM103-′yycH5
    pJC12pJM103-′yycI5
    pJC13pJM103-′yycJ5
    pJC14pJM103-′yycK5
    pYOCHPpJM115-PYocH-lacZThis work
    pJS01pJM103-yycH′-′phoAThis work
    pJS02pMA5-yycH′-′phoAThis work
    pJS03pJM117-′yycHThis work
    pJS04pJM117-′yycH′ ΔlacZThis work
    pJS05pJM134-′yocHThis work
Strains
    E. coli
        DH5αCloning hostLab stock
        TG1Cloning hostLab stock
    B. subtilis
        MH3402pheA1 trpC2 phoA::cat phoB::mTn10F. M. Hulett
        JH642pheA1 trpC2Lab stock
        JH17038pheA1 yycFH215PThis work
        JH17041leu-8 met-5 yycFH215P5
        JH25001amyE::(PyocH-lacZ aph3-A)This work
        JH25002yycH::pJC11 amyE(PyocH-lacZ aph3-A)This work
        JH25003yycI::pJC12 amyE(PyocH-lacZ aph3-A)This work
        JH25004yycJ::pJC13 amyE(PyocH-lacZ aph3-A)This work
        JH25005yycK::pJC14 amyE(PyocH-lacZ aph3-A)This work
        JH25011yycH::pJS03 amyE(PyocH-lacZ aph3-A)This work
        JH25012yocH::pJM134 amyE(PyocH-lacZ aph3-A)This work
        JH25013yocH::pJM134 yycH::pJC11 amyE(PyocH-lacZ aph3-A)This work
        JH25014phoA::cat phoB::mTn10 pJS04This work