TABLE 1.

Bacterial strains used in this study

Strain or plasmidRelevant property(ies)Reference
S. aureus strains
    V329Bovine clinical isolate, bap positive22
    c104Bovine clinical isolate, bap positive40
    V858Bovine clinical isolate, bap positiveThis study
    RN4220Restriction-deficient mutant of 8325-424
    CYL316Recipient strain for plasmid pCL8425
    SH1000Functional rsbU derivative of 8325-422
    NewmanNatural isolate18
    PC18398325-4 sarA::km8
    ISP5468325-4 agr::Tn55132
    AK18325-4 aur::ermB23
    AK28325-4 ssp::ermB23
    JP60Newman with the bap gene from the V858 strain inserted in its genomeThis study
    JP61SH1000 with the bap gene from the V858 strain inserted in its genomeThis study
    JP62V329 ΔsarA41
    JP63c104 ΔsarAThis study
    JP64JP60 sarA::kmThis study
    JP65JP61 sarA::kmThis study
    JP66JP62 pCU1-sarAThis study
    JP67JP63 pCU1-sarAThis study
    JP68JP64 pCU1-sarAThis study
    JP69JP65 pCU1-sarAThis study
    JP70JP62 pCU1This study
    JP71JP63 pCU1This study
    JP72JP64 pCU1This study
    JP73JP65 pCU1This study
    JP74JP64 aur::ermBThis study
    JP75JP64 ssp::ermBThis study
    JP76JP65 aur::ermBThis study
    JP77JP65 ssp::ermBThis study
    JP78JP61 pJP21This study
    JP79JP65 pJP21This study
    JP80V329 Δagr41
    JP81JP60 agr::ermBThis study
    JP82JP61 agr::ermBThis study
E. coli strains
    DH5αLaboratory strain
Plasmids
    pCL84Integrating plasmid25
    pJP17pCL84 carrying the bap gene under the the control of its native promoterThis study
    pALC2485Plasmid for construction of luxABCDE fusionsA. L. Cheung
    pJP21bap promoter-luxABCDE fusion formed by cloning the bap promoter region in plasmid pALC2485This study
    pCU1Shuttle plasmid2
    pCU1-sarApCU1 carrying sarA41