TABLE 1

Pupylated proteins identified in S. coelicolor

S. coelicolor gene locusProtein nameAnnotationDetected pupylated peptide(s)aPupylated lysineMediumbOrthologuec in:Search engine(s)dCOG category
M. tuberculosisM. smegmatisR. erythropolisC. glutamicum
SCO1814InhA/FabIPutative enoyl-(acyl carrier protein) reductase(R)DLGK(+243)QNIR(C)K177RRv1484MSMEG_3151RER_30750cg2342MII
SCO2090FtsICell division protein(K)TGTANRVDPATGK(+243)(Y)K582RRv2163cMSMEG_4233RER_35580cg2375MM
SCO2318Putative glycosyl transferase(R)TEHPRAHLVVVGK(+243)(R)K293RMSMEG_6484cg0481MM
SCO2389AcpPAcyl carrier protein(M)AATQEEIVAGLAEIVNEIAGIPVEDVK(+43)LDK(+243)(S)K31YRv2244MSMEG_4326RER_36730cg2473MII
SCO2390FabF3-Oxoacyl-(acyl carrier protein) synthase II(R)K(+243)VLGDDVDHM(+16)AVSGTK(S)K333RRv2246MSMEG_4328RER_36740cg3178MII
SCO2950HupANucleoid-associated heat-unstable (HU) protein(K)DADAVLAAFAEVVGDIVSK(+43)GDEK(+243)(V),K38YRv2986cMSMEG_2389MIL
(K)DADAVLAAFAEVVGDIVSK(+243)GDEK(V)
(K)DADAVLAAFAEVVGDIVSKGDEK(+243)(V)K42
SCO3373ClpCClp family ATP-binding protease(K)IAGVRRDK(+243)(E)K431RRv3596cMSMEG_6091RER_06460cg3079MO
SCO3581Conserved hypothetical protein(K)IVNPK(+243)VAGQAEGGR(V)K134YRv3688cMSMEG_6214RER_04620cg0335MIS
SCO3629PurAAdenylosuccinate synthetase(K)IGTTGRGIGPTYADK(+243)(I)K139RRv0357cMSMEG_0759RER_14280cg3063MF
SCO3878DnaNDNA polymerase III, beta chain(R)EFLWK(+243)PENPDISAVALVPAK(T)K182YRv0002MSMEG_0001RER_00020cg0004MIL
SCO4296GroEL260-kDa chaperonin 2(K)VTLGPKGRNVVLEK(+243)(K)K41R/YRv3417cMSMEG_1583RER_19240cg3011MO
SCO4496Putative acetyltransferase(R)FYESC(+57)GYK(+243)EVGRVPGAIR(V)K147R/YMIK
SCO4662Tuf1Elongation factor Tu-1(R)TVGAGQVTKINK(+243)(−)K397RRv0685MSMEG_1401RER_17640cg0587MIJ
SCO4762GroEL160-kDa chaperonin(K)DDTTIVDGAGK(+243)RDEVQGR(I)K338YRv0440MSMEG_0880RER_15260cg3011MIO
(K)GQGFNAATGEYGDLVK(+243)AGVIDPVK(V)K490
SCO5032AhpCAlkyl hydroperoxide reductase(K)GKEFQQINHK(+243)TYEGQWK(V)K31R/YRv2428MSMEG_4891RER_27870cg2736MIO
SCO5679Putative aldehyde dehydrogenase(R)EAVDAGAK(+243)LLTGGK(R)K351RMSMEG_2163RER_44340cg0067MC
SCO5748OsaAPutative sensory histidine kinase(K)GDLSKKITVDAK(+243)(G)K813Rcg0483MT
SCO6042Conserved hypothetical protein(R)AGYADEVQELFDQLAAK(+243)DVTIR(G)K62YRv2676cMSMEG_2782RER_28490cg2079MIS
SCO7343HemC1Porphobilinogen deaminase 1(R)K(+243)LTDLPR(G)K113RRv0510MSMEG_0953RER_16420cg0498MIH
SCO7442Putative uncharacterized protein(K)AVSMSDPNKDTNK(+243)(K)K22RMS
  • a Amino acid modifications are indicated as follows: +16, oxidation; +57, carbamidomethyl; +43, carbamyl; +243, pupylation.

  • b Y, YEME medium; R, R2YE medium.

  • c Orthologous proteins detected as confirmed pupylated targets in mycobacterial, Rhodococcus, or Corynebacterium pupylomes (10, 11, 19, 25, 50) are underlined. Orthologous proteins identified in these organisms were obtained by a BLAST search through the MAGE genome browser (https://www.genoscope.cns.fr/agc/microscope/home/index.php).

  • d The search engine used was Mascot (M), InSpecT (I), or both (MI).