TABLE 2.

Comparison of the putative P. putida PCL1445 grpE and dnaK heat-shock promoter sequences with promoters from E. coli, P. syringae pv. glycinea, and C. crescentus

Promoter (reference)−35 region sequenceSpacing (bp)−10 region sequence
E. coli σ32 consensus (16)TCTC-CCCTTGAA13-15 CCCCAT TTA
P. syringae pv. glycinea dnaK (16)GAGCAGGCTTGAA13 CCCCAT TTA
Caulobacter crescentus dnaK P1 (8)TTATGGCCTTGCG13 CCCCAT ATC
P. putida PCL1445
    grpE (P1)TGCCCCTTGAA14 CCCCAT ATA
    dnaK (P2)AAAGGCTTGAA14 CCCCAT CTA