TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
    Pseudomonas
        PCL1445Wild-type P. putida; colonizes grass roots and produces biosurfactants 23
        PCL1436Tn5luxAB derivative of PCL1445; mutated in psoA, a lipopeptide synthetase homologue 26
        PCL1622Tn5luxAB derivative of PCL1445; mutated in a gacA homologueThis study
        PCL1623Tn5luxAB derivative of PCL1445; mutated in a gacS homologueThis study
        PCL1627Tn5luxAB derivative of PCL1445; mutated in a dnaK homologueThis study
        PCL1628PCL1445 derivative mutated in the dnaJ homologue; constructed by single homologous recombinationThis study
        PCL1629PCL1445 derivative mutated in the grpE homologue; constructed by single homologous recombinationThis study
    Escherichia coli
        DH5α EndA1 gyrSA96 hrdR17 (rK mK) supE44 recAI; general purpose host strain used for transformation and propagation of plasmids 11
Plasmids
    pRL1063aPlasmid harboring a promotorless Tn5luxAB transposon, Kmr 48
    pRK2013Helper plasmid for triparental mating, Kmr 42
    pMP5505pRL1063a-based plasmid recovered from chromosomal DNA of PCL1627 after digestion with EcoRI with Tn5luxAB, KmrThis study
    pME6010Cloning vector which is maintained in Pseudomonas strains without selection pressure, Tcr 12
    pME3049Cloning vector, used for homologous recombination, Tcr Hgr 8
    pML103pML10 derivative lac-fusion broad-host-range vector for gram-negative bacteria, Gmr 27
    pJBA89pUC18 Not-luxR-PluxIPBSII-gfp (ASV)- To -T1, Apr 1
    pBBR1MCS-5Broad-host-range cloning vector for gram-negative bacteria, Gmr 23
    pMP4669pME6010 derivative harboring Ptac DsRed, Tcr 4
    pMP6562pME6010 containing gacS gene of PCL1171, used for complementation, Tcr 45
    pMP5285pME3049 derivative, missing the Hgr gene, used for single homologous recombination, Kmr 25
    pMP5512pMP6010 containing a PCR fragment of 1.3 kb with gacA gene of PCL1445, TcrThis study
    pMP5518pME6010 containing a PCR fragment of 3.5 kb with the dnaK and dnaJ genes from pMP5505, used for complementation, TcrThis study
    pMP5519pMP5518 derivative containing dnaK gene and the 5′ 366 bp of dnaJ gene, TcrThis study
    pMP5530pMP5518 containing the 3′ 520 bp of dnaK and dnaJ gene TcrThis study
    pMP5534pME6010 containing a PCR fragment of 1.1 kb with the grpE gene from pMP5505, TcrThis study
    pMP5524pMP5285 containing a 0.6 kb EcoRI-EcoRI PCR fragment of the central part of dnaJ gene from pMP5505, KmrThis study
    pMP5532pMP5285 containing a 0.5 kb EcoRI-EcoRI PCR fragment of the central part of grpE gene from pMP5505, KmrThis study
    pMP5535pML103 containing the dnaK::lacZ promoter in transcriptionally active orientation, GmrThis study
    pMP5536pML103 containing the dnaK::lacZ promoter in transcriptionally inactive orientation, GmrThis study
    pMP5537pMP6516 derivative with phzA promoter replaced by psoA promoter in transcriptionally active orientation, GmrThis study
    pMP5538pMP6516 derivative with phzA promoter replaced by psoA promoter in transcriptionally inactive orientation, GmrThis study
    pMP5539pMP5537 derivative harboring psoA::gfp transcriptionally active fused to pMP4669 harboring Ptac DsRed, Gmr TcrThis study
    pMP5540pMP5538 derivative harboring psoA::gfp transcriptionally inactive fused to pMP4669 harboring Ptac DsRed, Gmr TcrThis study
    pMP7551pGemT cloning vector containing an amplified cDNA fragment of 0.75 kb with the beginning part of dnaJ, CbrThis study