TABLE 3.

Proteins induced two-fold or more in protein extracts from cells growing in the presence of toluene

SpotProteinGeneAccession no.aProtein mol wt (103)n-Fold increase
19Toluene 2,3-dioxygenase (α subunit)todC1PPUY1824551.33.85
2402-Hydroxy-2,4-dienoate hydratasetodGPPUY1824528.324.75
2182-Hydroxy-6-oxo-2,4-heptadienoate hydrolasetodFPPUY1824530.82.41
78Arginine deiminasearcAPP100146.85.72
67Arginine deiminasearcAPP100146.86.9
2893-Isopropylmalate dehydratase, small subunitleuDPP198624.32.72
2765,10-Methylene-tetrahydrofolate dehydrogenase/cyclohydrolasefolD2PP226530.96.3
471Phosphoribosylformylglycinamide cyclo-ligasepurMPP166537.42.82
168GlucokinasegckPP101134.23.35
93Xenobiotic reductase A/xenobiotic flavin oxidoreductase AxenAPP125439.92.6
107Translation elongation factor Tutuf-1PP044043.83.54
90Sugar ABC transporter, periplasmic sugar-binding proteinPP101545.55.75
392Heat shock protein 10 (Cpn10)groESPP136010.23.9
  • a PP numbers are based on the locus of the genome of Pseudomonas putida KT2440. PPUY numbers are the accession numbers of the TodC1, TodG, and TodF nucleotide sequences of P. putida DOT-T1E deposited in GenBank.