TABLE 2.

Spots only present in gels from extracts of cultures grown in the presence of toluene

Spot(s)ProteinGeneAccession no.aProtein mol wt (103)
638, 636, 1184, 1180Toluene 2,3-dioxygenase (α subunit)todC1PPUY1824551.3
13392-Hydroxy-6-oxo-2,4-heptadienoate hydrolasetodFPPUY1824530.8
603Succinate-semialdehyde dehydrogenasegabDPP021351.8
1227Succinyl-coenzyme A synthetase (β subunit)sucCPP418641.5
563Oxoglutarate dehydrogenase, lipoamide dehydrogenase componentlpdGPP418750.1
1232Fructose-1,6-bisphosphate aldolasefdaPP496038.7
1140Phosphoenolpyruvate carboxykinase (ATP)pflu3720bPflu372058.3
1401Inorganic pyrophosphataseppaPP053819.1
1808, 1222, 1203, 1209Translation elongation factor Tutuf-1PP044043.8
1238ABC transporter, periplasmic binding proteinPP172638.6
1480Cold shock protein CspAcspA2PP24637.7
  • a PP numbers are based on the locus of the genome of Pseudomonas putida KT2440. PPUY numbers are the accession numbers of the TodC1 and TodF nucleotide sequences of P. putida DOT-T1E deposited in GenBank.

  • b Based on the protein identified in Pseudomonas fluorescens PfO-1.