TABLE 1

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionSource or reference
E. coli strains
    DH5αrecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR Δ(lacZYA-argF)U169 λ (ϕ80dlacZΔM15)Laboratory collection, CGSC 12384
    S17-1recA pro hsdR RP4-2-Tc::Mu-Km::Tn718
R. sphaeroides strains
    2.4.1Wild typeATCC 17023
    CL32.4.1 with kanamycin resistance cassette in place of genomic cls26
Plasmids
    pIND4IPTG-inducible expression vector for R. sphaeroides, kanamycin resistant17
    pIND4spVariant of pIND4 with spectinomycin resistance cassette in place of kanamycin resistance cassetteB. Burger and R. Lemke
    pIND5spDerivative of pIND4sp with NcoI site replaced with NdeI siteThis study
    cls-pIND5sppIND5sp containing clsThis study