TABLE 2.

Phenotypic analysis of mutations giving rise to defects in biofilm formation

ClassMutated geneaFunction and/or cellular locationMotilitybMean cell length (μm)c
SwimmingSwarmingBrothAgar
IflhDMaster regulator1.7 ± 0.12.1 ± 0.1
IIfliFMS ring2.2 ± 0.258.9 ± 11.8
fliGMotor switch33.8 ± 10.645.0 ± 9.4
fliLHook basal body35.4 ± 7.769.3 ± 22.9
fliMC ring+/−1.9 ± 0.32.0 ± 0.2
fliPExport pathway++1.9 ± 0.12.2 ± 0.3
fliQExport pathway1.9 ± 0.22.0 ± 0.2
flhAExport pathway1.7 ± 0.11.9 ± 0.2
IIIflgKHook junction1.8 ± 0.21.9 ± 0.3
flgLHook junction1.9 ± 0.11.9 ± 0.2
flgBBasal body rod2.0 ± 0.22.3 ± 0.2
  • a Based on BLAST protein:protein homology to sequences in the P. mirabilis HI4320 genome database (ftp://ftp.sanger.ac.uk/pub/pathogens/pm/).

  • b Motility is scored on a relative scale from − to ++++, where − indicates no motility and ++++, indicates wild-type motility.

  • c Mean cell length (± standard error of the mean) as determined using light-microscopic measurements of 50 cells for each determination. The mean cell length of wild-type swarmer cells obtained from the periphery of a swarming colony is 45.3 ± 9.1 μm, while the mean swimmer-cell length is 1.9 ± 0.3 μm.