TABLE 6.

Antibiotic resistance and biochemical characteristics of the mutant strains of S. haemolyticus JCSC1435

Strain or speciesMIC (mg/literb)Biochemical characteristicc
TEICVANCZXAMPMNETREGLSLACSACONPGMALMANGLNGLYFRUARGSALRAFUREVPNITDNACode no.Determination by code no.
JCSC1435642>12864++++++++++2-2-7-7-0-3S.haemolyticus
WP12a322>12864++++++++++2-2-7-7-0-3S.haemolyticus
3DC1a32220.25++++++++2-3-5-3-0-2Not determined
7BC164241+++++++++++2-3-7-7-0-3S.haemolyticus
4FT181>12864++++++++++2-3-7-3-0-3Not determined
8HT4a81>12832++++++++++2-3-7-3-0-3Not determined
7GC116241+++++++++++2-3-7-7-0-3S.haemolyticus
8GC1a32241++++++++++2-3-7-3-0-3Not determined
4IA1a41>12864++++++++++2-3-7-3-0-3Not determined
7HA164241+++++++++++2-3-7-7-0-3S.haemolyticus
8FCA116241++++++++++2-3-7-3-0-3Not determined
S. haemolyticusd+dd++d+NDd+ddds
  • a Chromosomal deletion sites of this strain is indicated in Fig. 5b.

  • b Alteration of MIC from JCSC1435 is indicated in bold. Tested antibiotics were teicoplanin (TEIC), vancomycin (VAN), ceftizoxime (CZX), and ampicillin (AMP).

  • c Biochemical reactions were tested by STAPHYOGRAM. Alteration of a reaction from that of JCSC1435 is indicated in bold. Tested reactions were production of an acid from mannose (MNE), an acid from trehalose (TRE), beta-glucosidase (GLS), an acid from lactose (LAC), an acid from saccharose (SAC), beta-galactosidase (ONPG), an acid from maltose (MAL), an acid from mannitol (MAN), beta-glucuronidase (GLN), an acid from glycerol (GLY), an acid from fructose (FRU), hydrolyzation of arginine (ARG), production of an acid from salicin (SAL), an acid from raffinose (RAF), urease (URE), production of acetoin (VP), reduction of nitrate (NIT), and hydrolyzation of DNA (DNA).

  • d Definition of S. haemolyticus, cited from Bergey's manual, for reference. +, more than 90% of strains are positive; −, less than 10% of strains are positive; d, 11 to 89% of strains are positive; ds, test differentiates subspecies (not separated out above in heading); ND, test not determined.