TABLE 1.

Bacterial strains and plasmids

Strain/plasmidGenotypeReference/source
E. coli strain SM10λpir thi thr leu tonA lacY supE recA::RP4-2 Tc::MuλpirR6K; Kmr 51
V. cholerae strains
    PW249MO10, clinical isolate of V. cholerae O139 from India, Smr 70
    PW357MO10lacZ::vpsLplacZ, Smr 25
    PW396MO10, ΔvpsA-vpsK, Smr 40
    PW444MO10lacZ::vpsLplacZ, ΔmbaA, SmrThis study
    PW512MO10lacZ::vpsLplacZ, ΔVC0702, SmrThis study
    PW514MO10lacZ::vpsLplacZ, ΔVC0704, SmrThis study
    PW522MO10lacZ::vpsLplacZ, ΔVC0704ΔmbaA, SmrThis study
Plasmids
    pWM91 oriR6K mobRP4 lacI Ptac tnp mini-Tn10Km; Kmr, Apr 49
    pACYC184Kmr Cmr 6
    pCR2.1-TOPOPlasmid for TOPO cloning, AprInvitrogen
    PK105pWM91 carrying an internal in-frame deletion of VC0704This study
    PK104pWM91 carrying an internal in-frame deletion of VC0702This study
    pNB4pWM91 carrying an internal in-frame deletion of mbaA 3