TABLE 2.

Primers

ConstructPrimerDescriptionSequence
V. cholerae VC0702 deletionP200Forward primer for upstream fragment 15′-GGAACTCTCGCGTCTTGGCTATG-3′
P201Reverse primer for upstream fragment5′-GCCGCAGCGGCCGCAGGTGCTAAAGGCACTTCTAACG-3′
P202Forward primer for downstream fragment5′-TGCGGCCGCTCGGGCGCTCTGATCCCCTTTATTAATCCA-3′
P203Reverse primer for downstream fragment 15′-TCCTTCTTTTAGTGAGTGGCAGCAAG-3′
P208Forward primer for upstream fragment 25′-TCGAGGAACTCTCGCGTCTTGGCTATG-3′
P209Reverse primer for downstream fragment 25′-CTAGTCCTTCTTTTAGTGAGTGGCAGCAAG-3′
V. cholerae VC0704 deletionP204Forward primer for upstream fragment 15′-GATATGACTAAGCTGGAATCGCGTC-3′
P205Reverse primer for upstream fragment5′-GCCCGAGCGGCCGCACAGCGACAGAAAGCGTTTGATC-3′
P206Forward primer for downstream fragment5′-TGCGGCCGCTCGGGCCTCAGTCTGCGTGCCAAAATCATC-3′
P207Reverse primer for downstream fragment 15′-TTGGCCATTGAGGATCGATAACG-3′
P210Forward primer for upstream fragment 25′-TCGAGATATGACTAAGCTGGAATCGCGTC-3′
P211Reverse primer for downstream fragment 25′-CTAGTTGGCCATTGAGGATCGATAACG-3′