TABLE 3.

Regulation of rctBp activity by Dam

lacZ fusionArabinoseaβ-Galactosidase activity (MU) inRelative promoter activity (Dam/Dam+)
CVC73 (Dam+)CVC72 (Dam)
pDP309 (rctBp-lacZ)5,153 ± 478 (1)4,055 ± 652 (1)0.79
+2,840 ± 157 (0.55)b1,634 ± 300 (0.40)0.58
pDP336 (rctBp-lacZ)6,525 ± 268 (1)3,575 ± 141 (1)0.55
+4,517 ± 112 (0.69)1,653 ± 285 (0.46)0.37
pPP112 (repAp-lacZ)2,300 ± 36 (1)2,094 ± 16 (1)0.91
+2,283 ± 11 (0.99)1,978 ± 33 (0.94)0.87
  • a To induce RctB from pBADrctB present in pTVC11).

  • b Relative promoter activity after RctB induction.