TABLE 1.

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerRelevant characteristic(s)a or sequenceReference or source
P. aeruginosa strains
    PA14Wild type 48
    PA14 mutantsPA14 flgK::Tn5B30; Tcr 42
PA14 rhlA::Gm; Gmr 46
PA14 rhlB::Kn; KnrThis study
PA14 ΔrhlCThis study
PA14 sadB::Tn5B21; Tcr 7
PA14 ΔpqsDD. Hogan
Plasmids
    pNC5 sadB in pUCP18; Cbr Apr 7
    pEX18GmSuicide vector; Gmr Sac+ 25
    pEX18KOrhlC rhlC KO constructThis study
    pBT20Transposon plasmid; Gmr 33
Primers
    rhlCKO15′-GGCGTCTAGACTGGCGATGCTCGGCTTC-3′
    rhlCKO25′-GGAGGAATTCCAGGTCGTCGTCGCCAG-3′
    rhlCKO35′-GGCGGAATTCCGGCGAAACGCATTC-3′
    rhlCKO45′-GGCGAAGCTTCTGGAACTTTCGGTG-3′
  • a Abbreviations: Tcr, tetracycline resistant; Gmr, gentamicin resistant; Knr, kanamycin resistant; Cbr, carbenicillin resistant; Apr, ampicillin resistant; KO, knockout.