TABLE 1.

Strains, plasmids, and primers used in this study

Strain, plasmid, or primerRelevant characteristic(s)a or sequenceReference or source
P. aeruginosa strains
    PA14Wild type48
    PA14 mutantsPA14 flgK::Tn5B30; Tcr42
PA14 rhlA::Gm; Gmr46
PA14 rhlB::Kn; KnrThis study
PA14 ΔrhlCThis study
PA14 sadB::Tn5B21; Tcr7
PA14 ΔpqsDD. Hogan
Plasmids
    pNC5sadB in pUCP18; Cbr Apr7
    pEX18GmSuicide vector; Gmr Sac+25
    pEX18KOrhlCrhlC KO constructThis study
    pBT20Transposon plasmid; Gmr33
Primers
    rhlCKO15′-GGCGTCTAGACTGGCGATGCTCGGCTTC-3′
    rhlCKO25′-GGAGGAATTCCAGGTCGTCGTCGCCAG-3′
    rhlCKO35′-GGCGGAATTCCGGCGAAACGCATTC-3′
    rhlCKO45′-GGCGAAGCTTCTGGAACTTTCGGTG-3′
  • a Abbreviations: Tcr, tetracycline resistant; Gmr, gentamicin resistant; Knr, kanamycin resistant; Cbr, carbenicillin resistant; Apr, ampicillin resistant; KO, knockout.