TABLE 3.

PospA-gfp fluorescence levels from spirochetes grown at 37°C and within DMCs

CloneBackgroundPromoter37°C in vitroDMC
MFI% FluoraMFI% Fluor
CE56wt rpoSflaB239.679.9395.982.1
CE103wt rpoSospA958.086.411.767.8b
CE472rpoS::ermCospA2,547.490.11,838.580.2
  • a % Fluor, percent fluorescence determined by flow cytometry, confirmed visually using fluorescence microscopy.

  • b At least 60% of the recovered spirochetes retained the shuttle vector as determined by solid-phase plating in the presence and absence of the selecting antibiotic.