TABLE 1.

B. subtilis genes with LexA binding sites in their putative promoter regions

GeneaOperator sequencebPositioncKdd (nM)Fold inductione
    ybaKaGAACATtTGTTCc−126 (+15)6.52.3
        cwlD2.1
dinBaGAACtcATGTTCG−42 (−14)3.655
ydgGCGAACATgaGTTCt−114 (−63)3.61.4
        ydgH1.2
    ydiOaGAACATtcGTTCt−51 (+13)163.5
        ydiP3.6
    pcrAaGAACgTATGTTtt−22 (+8)222.0
        ligA2.0
        yerHND
    yhaZaGAACgTAcaTTCc−39 (−15)2011
    yhaOaGAACgTgcaTTCG−50 (−27)293.5
        yhaN1.6
        yhaM2.0
yhjDaGAACAaAcGTTCc−21 (+14)7.716
        yhjC2.9
        yhjB3.8
yhjEgGAACgTtTGTTCt−119 (−60)7.71.2
    xkdAaGAACAcAcGTTCG−156.13.8
    ykvRCGAACgTATGTTtG−111 (−64)131.4
    recACGAAtATgcGTTCG−73 (−44)4.69.4
    aprXCGAACAaAcGTTCt−166 (+9,−92)3.92.7
    lexA (1)gGAAtgTtTGTTCG−125 (−99)5.73.4
    lexA (2)CGAACAaAcGTTtc−88 (−62)
lexA (3)CGAACcTATGTTtG−59 (−33)
yneA (1)CaAACATAgGTTCG−49 (−44)5.737
    yneA (2)gaAACgTtTGTTCG−15 (−15)
    yneA (3)CGAACAaAcaTTCc−12 (+23)
        yneB44
        ynzC8.1
    parECaAACATAcGTTCt−205 (+17)9.62.9
        parC3.0
    yozL (1)CGAACtTtTGTTCt−99 (−17)5.81.5
yozL (2)gGAACgTtTGTTCt−68 (+14)
        yozK1.4
        yobH1.2
yozM (1)aGAACAaAcGTTCc−127 (−74)5.81.3
    yozM (2)aGAACAaAaGTTCG−97 (−44)
    yorBaGAACActTGTTCc−62121.7
    yolC (1)aGAACAaAcGTTCt−127 (−74)3.91.2
yolC (2)aGAACAaAaGTTCG−97 (−44)
yolD (1)CGAACtTtTGTTCt−64 (−17)3.91.6
    yolD (2)aGAACgTtTGTTCt−34 (+14)
        uvrX1.4
    hbsgGAAtATtcGTTCG−280 (−53)32−1.1
    ypuDaGAACATAaaTTCG−1576.41.3
    yqjW (1)CGAACATActTTCG−43 (−15)4.13.5
yqjW (2)CGAACATAaGTTCt−15 (+14)
        yqjX5.7
        yqjY1.8
        yqjZ1.9
yqzH (1)aGAACtTATGTTCG−138 (−114)4.11.7
    yqzH (2)CGAAagTATGTTCG−110 (−86)
yqhBCaAACtTtTGTTCt−1301401.2
yqxLaGAACAaAaGTTtG−131.1
    sdaaGAACgatTGTTCt−113 (−60)101.7
    ruvACGAACATATGTTaa−65 (−45)672.2
        ruvB2.2
    uvrCaaAACAaAcGTTCG−45 (−16)401.6
    dnaEaGAACATtTGTTtc−62 (−32)391.2
    uvrBCGAACtTtaGTTCG−79 (−42)4.111
        uvrA12
    dinC (1)aGAACAagTGTTCt−85 (−44)2.3175
    dinC (2)CGAACgTATGTTtG−55 (−14)
    vprCGAACgTATaTTCc−177 (−55)6.81.1
Half site consensus sequenceC28G56A66A66C58A34T40
  • a Genes listed by map position. The first gene in operons is shown in boldface type; subsequent genes in putative multigene operons are shown indented. Arrows indicate divergently transcribed genes. The numbers in parentheses indicate different operators within the same promoter region.

  • b Lowercase nucleotides and uppercase nucleotides indicate nonconsensus and consensus, respectively.

  • c Location of the 3′ end relative to the ATG codon of the respective gene (and relative to the 3′ end of the −10 region of the canonical promoter sequence).

  • d Apparent binding constant.

  • e Fold induction following treatment with MC in wild-type cells; data also listed in Table 4.