TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
    Erwinia carotovora subsp. atroseptica Ecal2Wild type58
    Erwinia carotovora subsp. betavasculorum Ecb168Wild typeJ. E. Loper
    Erwinia carotovora subsp. carotovora
        Ecc71Wild type58
        AC5094AhlI derivative of Ecc717
        AC5111AhlI derivative of EC153This study
        SCC3193Wild type43
        SCRI193Wild type44
        EC153Wild typeLaboratory collection
    Escherichia coli
        DH5αφ80lacZΔM15 Δ(lacZYA-argF) U169 hsdR17 recA1 endA1 thi-1Gibco BRL
        MC4100araD139 Δ(lacIPOZYA)U169 recA1 thi-1 Strr27
        VJS533araD(lac-proAB) rpsL φ80 lacZΔM15 recA5620
Plasmids
    pDK6Kmr24
    pLARF5Tcr23
    pMAL-c2gApr, protein expression vectorNew England Biolabs
    pMP220Tcr, promoter-probe vector47
    pCL1920Spr Smr26
    pAKC781peh-1+ DNA in pBluescriptSK(+)27
    pAKC783Apr, pel-1+ DNA in pBluescriptSK(+)27
    pAKC856Apr, ahlI+ DNA in pBluescriptSK(+)7
    pAKC882Apr, rsmA coding region in pT7-738
    pAKC924Apr, hrpN+ in pBluescriptSK(+)14
    pAKC935Spr, expR3193+ in pCL1920This study
    pAKC936Spr, expR71+ in pCL1920This study
    pAKC937Spr, 0.8 kb expR153+ in pCL1920This study
    pAKC1034Apr, 200-bp celV fragment in pGEM-T Easy30
    pAKC1100Tcr, rsmA71-lacZ in pMP2206
    pAKC1101Tcr, rsmA71-lacZ in pMP220This study
    pAKC1102Tcr, rsmA71-lacZ in pMP220This study
    pAKC1103Tcr, rsmA71-lacZ in pMP220This study
    pAKC1104Tcr, rsmA71-lacZ in pMP220This study
    pAKC1106Tcr, rsmA153-lacZ, 0.4-kb rsmA153 upstream DNA in pMP220This study
    pAKC1201Kmr, ptac-ahlI71, ahlI71 coding region in pDK612
    pAKC1210Tcr, ahlI+ DNA of EC153 in pLARF5Laboratory collection
    pAKC1211Apr Tcr, AhlI derivative of pAKC1210Laboratory collection
    pAKC1221Apr, expR153 coding region in pMAL-c2gThis study
    pAKC1222Kmr, ptac-ahlI153, ahlI153 coding region in pDK6This study
    pHV200Apr, 8.8-kb SalI fragment containing lux operon20
    pHV200IApr, frameshift mutant of luxI in pHV200E. P. Greenberg
    pSB401Tcr, fusion of luxRl′::luxCDABE on pACYC184 plasmid backbone52