TABLE 1.

Strains used in this study

Strain or plasmidGenotypeReference/sourcea
Strains
    TR6583metE205 ara-9K. Sanderson via J. Roth
    Derivatives of TR6583
        JE2261zfa-3648* Tn10* zfa-3649eut)bLaboratory collection
        JE4175pBAD30 bla+Laboratory collection
        JE6692pKD46 bla+Laboratory collection
        JE7963eutE1151::cat+
        JE7973ΔeutE1164
        JE7979ΔeutE1164/pBAD30
        JE8032eutLK1152::cat+
        JE8033eutMN1153::cat+
        JE8064eutS1154::cat+
        JE8070ΔeutLK1158
        JE8071ΔeutMN1159
        JE8072ΔeutS1160
        JE8087eutA1155::cat+
        JE8088eutBC1156::cat+
        JE8089eutABC1157::cat+
        JE8093ΔeutA1161
        JE8094ΔeutBC1162
        JE8095ΔeutABC1163
        JE8102eutLK1152::cat+ ΔeutMN1159
        JE8103ΔeutLK1158/pBAD30
        JE8105ΔeutMN1159/pBAD30
        JE8133ΔeutLK1158 ΔeutMN1159
        JE8135ΔeutLK1158 ΔeutMN1159/pBAD30
        JE8138ΔeutABC1163/pEUT34
        JE8139ΔeutABC1163/pBAD33
        JE8142zfa-3648* Tn10* zfa-3649eut)/pBAD33, pTAC-85
        JE8144zfa-3648* Tn10* zfa-3649eut)/pEUT34, pTAC-85
        JE8191ΔeutE1164/pEUT40
        JE8217zfa-3648* Tn10* zfa-3649eut)/pEUT34, pEUT40
        JE8298pBAD33 pTAC-85
        JE8327zfa-3648* Tn10* zfa-3649eut)/pEUT42, pEUT40
        JE8392pBAD33 cat+
        JE8393ΔeutA1161/pEUT33
        JE8395ΔeutA1161/pBAD33
        JE8411ΔeutBC1162/pEUT42
        JE8412ΔeutBC1162/pBAD33
        JE8424pTAC-85 bla+
        JE8425ΔeutE1164/pTAC-85
        JE8426ΔeutS1160/pBAD30
    E. coli strain DH5αF f80ΔlacZDM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA115
Plasmids
    pBAD30bla+14
    pBAD33cat+14
    pET22-bbla+Novagen
    pTAC-85bla+20
    pEUT31eutBC+ in pET22-bG. Reed
    pEUT33eutA+ in pBAD33
    pEUT34eutABC+ in pBAD33
    pEUT40eutE+ in pTAC-85
    pEUT42eutBC+ in pBAD33
  • a Unless otherwise indicated, all strains were constructed during the course of this work.

  • b Asterisks indicate a Tn10-borne deletion.