TABLE 3.

Biofilm COMSTAT flow cell measurements for the addition of 6.4 μM AI-2 to ATCC 25404 and for the luxS (E. coli DH5α) and mqsR mutations

Strain and time after inoculation (h)Condition(s) or parameterBiomass (μm3/μm2)Substratum coverage (%)Mean thickness (μm)Roughness coefficient
ATCCNo AI-29 ± 341 ± 412 ± 20.9 ± 0.1
    25404 (16)6.4 μM AI-248 ± 1755 ± 1686 ± 130.3 ± 0.1
Ratio51.37−3.5
ATCCNo AI-215 ± 457 ± 725 ± 70.6 ± 0.2
    25404 (24)6.4 μM AI-268 ± 971 ± 794 ± 140.20 ± 0.08
Ratio4.51.23.7−2.9
DH5α (24)No AI-28 ± 341 ± 1110 ± 41.0 ± 0.2
MG1655 (24)No AI-225 ± 834 ± 1444 ± 60.2 ± 0.1
MG1655 ΔmqsR (24)No AI-25.6 ± 1.65 ± 227 ± 221.5 ± 0.4
MG1655 (48)No AI-235 ± 3045 ± 1054 ± 300.8 ± 0.6
MG1655 ΔmqsR (48)No AI-24.4 ± 72.4 ± 1.313 ± 51.7 ± 0.5