TABLE 1.

E. coli strains and plasmids useda

Strain or plasmidGenotypeSource
Strains
    Vibrio harveyi BB170BB120 luxN::Tn5 (AI-1 sensor, AI-2 sensor+)46
    E. coli K-12Wild typeATCC 25404
    E. coli DH5αluxS supE44 ΔlacU169 (φ80dlacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA136
    E. coli K-12 BW25113lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD7813
    E. coli K-12 BW25113 ΔluxSK12 ΔluxS::Kmr1
    E. coli JM109recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+ lacIq lacZΔM15]54
    E. coli K-12 MG1655F λ ilvG rfb-50 rph-15
    E. coli K-12 MG1655 ΔmotAΔmotA::Tn5Kan-223
    E. coli K-12 MG1655 ΔqseBΔqseB::Tn5Kan-223
    E. coli K-12 MG1655 ΔlsrKΔlsrK::Tn5Kan-223
    E. coli K-12 MG1655 ΔfliAΔfliA::Tn5Kan-223
    E. coli K-12 MG1655 ΔmqsRΔb3022::Tn5Kan-223
    E. coli W3110Wild type21
Plasmids
    R1drd19Ampr Kmr Cmr Smr; IncFII finO18
    pTrcHis-pfsAmpr; pTrcHisC (Invitrogen) with pfs from E. coli strain W311021
    pTrcHis-luxSAmpr; pTrcHisC (Invitrogen) with luxS from E. coli strain W311021
    pCM18Emr; pTRKL2-PCP25-RBSII-gfpmut3*-T0-T1 (GFP plasmid for visualizing biofilm)20
    pCA24NCmr; lacIq1
    pCA24N luxS+Cmr; lacIq; PT5-lac luxS1
    pVLT31 mqsR+pVLT31 plac::mqsR+This study
    pVS159Ampr; qseB::lacZ in pRS55145
    pVS176Ampr; motA::lacZ in pRS55145
    pVS175Ampr; fliC::lacZ in pRS55145
    pVS183Ampr; fliAehK12::lacZ in pRS55145
    pVS182Ampr; flhD::lacZ in pRS55145
  • a Ampr, Kmr, Cmr, Smr, Emr, Rifr, and Tcr, ampicillin, kanamycin, chloramphenicol, streptomycin, erythromycin, rifampin, and tetracycline resistance, respectively.