TABLE 2.

Transitions monitored in MRM experiments

AnalyteMass (m/z)Retention time (min)
Q1Q3
C4-HSL→lactone moiety172.2102.111.2
C4-HSL→acyl moiety172.271.211.2
3-Oxo-C4-HSL→lactone moiety186.2102.15.1
3-Oxo-C4-HSL→acyl moiety186.285.25.0
C5-HSL→lactone moiety186.2102.116.0
C5-HSL→acyl moiety186.285.216.0
3-Oxo C5-HSL→lactone moiety200.3102.19.6
3-Oxo-C5-HSL→acyl moiety200.399.29.5
C6-HSL→lactone moiety200.3102.120.0
C6-HSL→acyl moiety200.399.220.0
3-Oxo-C6-HSL→lactone moiety214.3102.113.9
3-Oxo-C6-HSL→acyl moiety214.3113.213.7
C7-HSL→lactone moiety214.3102.123.0
C7-HSL→acyl moiety214.3113.223.0
3-Oxo-C7-HSL→lactone moiety228.3102.117.9
3-Oxo-C7-HSL→acyl moiety228.3127.217.9
C8-HSL→lactone moiety228.3102.125.2
C8-HSL→acyl moiety228.3127.225.2
3-Oxo-C8-HSL→lactone moiety242.3102.121.3
3-Oxo-C8-HSL→acyl moiety242.3141.221.3
C9-HSL→lactone moiety242.3102.127.0
C9-HSL→acyl moiety242.3141.227.0
3-Oxo-C9-HSL→lactone moiety256.3102.123.8
3-Oxo-C9-HSL→acyl moiety256.3155.223.8
C10-HSL→lactone moiety256.3102.128.6
C10-HSL→acyl moiety256.3155.228.6
3-Oxo-C10-HSL→lactone moiety270.3102.125.8
3-Oxo-C10-HSL→acyl moiety270.3169.225.8
C11-HSL→lactone moiety270.3102.129.9
C11-HSL→acyl moiety270.3169.229.9
3-Oxo-C11-HSL→lactone moiety284.3102.127.5
3-Oxo-C11-HSL→acyl moiety284.3183.227.5
C12-HSL→lactone moiety284.3102.131.1
C12-HSL→acyl moiety284.3183.231.1
3-Oxo-C12-HSL→lactone moiety298.3102.129.0
3-Oxo-C12-HSL→acyl moiety298.3197.229.0
3-Hydroxy-C12-HSL→lactone moiety300.3102.129.2
3-Hydroxy-C12-HSL→acyl moiety300.3199.229.0
C13-HSL→lactone moiety298.3102.132.0
C13-HSL→acyl moiety298.3197.232.0
3-Oxo-C13-HSL→lactone moiety312.3102.130.3
3-Oxo-C13-HSL→acyl moiety312.3211.230.3
C14-HSL→lactone moiety312.3102.133.0
C14-HSL→acyl moiety312.3211.233.0
3-Oxo-C14-HSL→lactone moiety326.3102.131.3
3-Oxo-C14-HSL→acyl moiety326.3225.231.3
C15-HSL→lactone moiety326.3102.134.0
C15-HSL→acyl moiety326.3225.234.0
D3C6-HSL→lactone moiety203.3102.120.0
D3C6-HSL→acyl moiety203.3102.220.0