Strains of P. aeruginosa and E. coli and plasmids used in this study

Bacterial strain or plasmidGenotype or phenotypeSource or reference
    P. aeruginosa
        PAO1Wild type11
        PAO6261PAO1 Δanr49
        PAO-MW20PAO1 rpoS::aacC1 Gmr46
        PAO6251PAO1 ΔarcDABC17
        PAO6281PAO1 gacA::Ω-Sp/Sm36
        KS06PAO1 attB::(PPA3309-lacZ)This study
        KS08PAO6261 attB::(PPA3309-lacZ)This study
        KS10PAO-MW20 attB::(PPA3309-lacZ)This study
        KS11PAO1 attB::(mini-CTX-lacZ)This study
        KS15PAO1 attB::(PPA3309-gfp)This study
        KS16PAO1 ΔPA3309::aacC1 GmrThis study
        KS17PAO1 ΔPA3309This study
        KS27PAO1 attB::(mini-CTX2)This study
        KS28KS17 attB::(mini-CTX2)This study
        KS29KS17 attB::(pKS13)This study
        KS31PAO1 rhlR attB::(PPA3309-lacZ)This study
        KS32PAO1 lasR attB::(PPA3309-lacZ)This study
        KS33PAO6281 attB::(PPA3309-lacZ)This study
        KS35PAO1 ΔrelAThis study
        KS37PAO1 ΔrelA attB::(PPA3309-lacZ)This study
        NB015PAO1 ΔPA4352This study
    E. coli
        DH10BF mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu) 7697 galU galK λ rpsL nupGGibco BRL (Invitrogen)
        S17 λpirpro thi hsdR+ Tpr Smr; chromosome::RP4-2 Tc::Mu-Km::Tn7/λpir9
        SM10thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Kmr)9
        pEX18ApApr oriT+ sacB+; gene replacement vector with MCS from pUC1823
        Mini-CTX-lacZTcr; promoterless lacZ gene2
        Mini-CTX2Tcr; integration vector24
        pSB219.9AGmr; lasR::Gm suicide vector1
        pSB224.10ATcr; rhlR::Tc suicide vector1
        pPS858Apr Gmr; source of gentamicin cassette23
        pFLP2Apr; source of FLP recombinase23
        pMH305Apr Gmr; source of gfp cassetteMorten Hentzer
        pKS08Apr Gmr; pMH305 with 526 bp of PA3309 promoter between EcoRI and BamHIThis study
        pKS09Tcr; mini-CTX2 with NotI fragment liberated from pKS08 containing PA3309 promoter fused to gfpThis study
        pKS10Apr Gmr; pEX18Ap with 723-bp promoter of PA3309, Gmr-gfp fragment from pPS858 and 625 bp downstream of coding region of PA3309 between EcoRI and SalIThis study
        pKS13Tcr; mini-CTX2 with 1,200-bp PCR fragment covering entire PA3309 gene and 455 bp of putative promoter region and 289 bp of downstream region between EcoRI and HindIIIThis study
        pKS15Tcr; mini-CTX-lacZ containing 492-bp fragment of putative promoter region of PA3309 gene between EcoRI and BamHIThis study
        pKS18Apr Gmr; pEX18Ap with 796-bp promoter of relA (PA0934), Gmr-gfp fragment from pPS858, and 824 bp of the 3′ coding region of relA between EcoRI and HindIIIThis study
        pNB007Apr Gmr; pEX18Ap with 596-bp promoter PA4352, Gmr-gfp fragment from pPS858, and 617 bp downstream of coding region of PA4352 between SacI and HindIIIThis study